Australian propolis ethanol extract exerts antibacterial activity against methicillin-resistant Staphylococcus aureus by mechanisms of disrupting cell structure, reversing resistance, and resisting biofilm

文献类型： 外文期刊

第一作者： Wang, Fei

作者： Wang, Fei;Liu, Hui;Li, Junya;Xuan, Hongzhuan;Zhang, Wenwen;Jiang, Bin

作者机构：

关键词： Australian propolis; Methicillin-resistant Staphylococcus aureus; Cell structure; Drug resistance; Biofilm

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2021 年 52 卷 4 期

页码：

收录情况： SCI

摘要： The antibacterial activity and mechanisms of Australian propolis ethanol extract (APEE) against methicillin-resistant Staphylococcus aureus (MRSA) were investigated herein. The diameter of inhibition zones (DIZ) of APEE was 19.7 mm, while the minimum inhibition concentration (MIC) and minimum bactericide concentration (MBC) of APEE were both 0.9 mg/mL against the tested strain of MRSA. Nucleic acid leakage and propidium iodide (PI) staining assays showed that APEE can stimulate the release of intracellular nucleic acids by disrupting the integrity of the cell wall and cytoplasmic membrane. Scanning electron microscopy (SEM) further confirmed that APEE could depress cellular activities via damaging the cell structure, including the cell wall and membrane. Western blot analysis and beta-lactamase activity assay showed that APEE could inhibit the expression of PBP2a and reduce the activity of beta-lactamase, suggesting that APEE is able to reverse the drug resistance of MRSA. XTT and crystal violet (CV) assays indicated that APEE had the capacity to prevent the formation of biofilms through decreasing cellular activities and biomass. Bacterial adhesion assay revealed that APEE could reduce the adhesive capacity of the strain, belonging to its antibiofilm mechanisms. Furthermore, nine main compounds of APEE were identified and quantified by HPLC-DAD/Q-TOF-MS. The results above all verified that the antibacterial activity of APEE against MRSA was mainly due to disrupting cell structure, reversing resistance, and resisting biofilm formation, which indicates that APEE is expected to be an efficient functional ingredient with great potential application in the field of medicine and food.

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