Lactobacillus rhamnosus GG triggers intestinal epithelium injury in zebrafish revealing host dependent beneficial effects

文献类型： 外文期刊

第一作者： Zhang, Zhen

作者： Zhang, Zhen;Ran, Chao;Yang, Ya-Lin;Yao, Yuan-Yuan;Ding, Qian-Wen;Zhang, Zhen;Guan, Le-Luo;Zhang, Hong-Ling;Hao, Qiang;Yang, Hong-Wei;Meng, De-Long;Liu, Shu-Bin;Teame, Tsegay;Gao, Chen-Chen;Zhou, Zhi-Gang;Yang, Da-Hai;de Vos, Willem Meindert;de Vos, Willem Meindert;Teame, Tsegay

作者机构：

关键词： intestinal mucosal damage; Lactobacillus rhamnosus GG; probiotic safety; SpaC; zebrafish

期刊名称：IMETA （ 影响因子：33.2； 五年影响因子：33.2 ）

ISSN： 2770-5986

年卷期： 2024 年 3 卷 2 期

页码：

收录情况： SCI

摘要： Lactobacillus rhamnosus GG (LGG), the well-characterized human-derived probiotic strain, possesses excellent properties in the maintenance of intestinal homeostasis, immunoregulation and defense against gastrointestinal pathogens in mammals. Here, we demonstrate that the SpaC pilin of LGG causes intestinal epithelium injury by inducing cell pyroptosis and gut microbial dysbiosis in zebrafish. Dietary SpaC activates Caspase-3-GSDMEa pathways in the intestinal epithelium, promotes intestinal pyroptosis and increases lipopolysaccharide (LPS)-producing gut microbes in zebrafish. The increased LPS subsequently activates Gaspy2-GSDMEb pyroptosis pathway. Further analysis reveals the Caspase-3-GSDMEa pyroptosis is initiated by the species-specific recognition of SpaC by TLR4ba, which accounts for the species-specificity of the SpaC-inducing intestinal pyroptosis in zebrafish. The observed pyroptosis-driven gut injury and microbial dysbiosis by LGG in zebrafish suggest that host-specific beneficial/harmful mechanisms are critical safety issues when applying probiotics derived from other host species and need more attention.

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