A Point-of-Care Testing Device Utilizing Graphene-Enhanced Fiber Optic SPR Sensor for Real-Time Detection of Infectious Pathogens

文献类型： 外文期刊

第一作者： Jiang, Shiyu

作者： Jiang, Shiyu;Qian, Siyu;Zhu, Shunning;Lu, Jinxin;Hu, Yunxin;Geng, Yikai;Chen, Xuefeng;Guo, Ying;Liu, Shengchun;Jiang, Shiyu;Hu, Yunxin;Zhang, Cheng;Chen, Zhaoliang;Pu, Jie;Guo, Zhendong

作者机构：

关键词： surface plasmon resonance; infectious pathogen detection; portable device; graphene film; point-of-care testing

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN：

年卷期： 2023 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Timely detection of highly infectious pathogens is essential for preventing and controlling public health risks. However, most traditional testing instruments require multiple tedious steps and ultimately testing in hospitals and third-party laboratories. The sample transfer process significantly prolongs the time to obtain test results. To tackle this aspect, a portable fiber optic surface plasmon resonance (FO-SPR) device was developed for the real-time detection of infectious pathogens. The portable device innovatively integrated a compact FO-SPR sensing component, a signal acquisition and processing system, and an embedded power supply unit. A gold-plated fiber is used as the FO-SPR sensing probe. Compared with traditional SPR sensing systems, the device is smaller size, lighter weight, and higher convenience. To enhance the detection capacity of pathogens, a monolayer graphene was coated on the sensing region of the FO-SPR sensing probe. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was used to evaluate the performance of the portable device. The device can accurately detect the SARS-CoV-2 spike S1 protein in phosphate-buffered saline (PBS) and artificial saliva within just 20 min, and the device successfully detected cultured SARS-CoV-2 virus. Furthermore, the FO-SPR probe has long-term stability, remaining stable for up to 8 days. It could distinguish between the SARS-CoV-2 spike protein and the MERS-CoV spike protein. Hence, this FO-SPR device provides reliable, rapid, and portable access to test results. It provides a promising point-of-care testing (POCT) tool for on-site screening of infectious pathogens.

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