Integrated transcriptomics and metabolomics elucidate additive inhibitory effects of combined salinity-waterlogging stress on soybean growth and metabolic adaptations

文献类型： 外文期刊

第一作者： Qiu, Shen

作者： Qiu, Shen;Zhang, Yanjun;Sun, Hongchun;Liu, Liantao;Li, Cundong;Dong, Hezhong;Qiu, Shen;Zhang, Yanjun;Hua, Ziqing;Dong, Hezhong

作者机构：

关键词： Soybean ( Glycine max ); Combined salinity-waterlogging stress; Additive inhibition; Transcriptomic-metabolomic integration; Ionic homeostasis; Flavonoid metabolism

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 223 卷

页码：

收录情况： SCI

摘要： Salinity and waterlogging are major abiotic stresses constraining soybean productivity, with their combined effects often surpassing individual impacts. However, the nature of these combined effects-whether additive, synergistic, or antagonistic-remains unclear. In this study, we quantitatively demonstrated that combined salinity-waterlogging stress exerts additive inhibitory effects on soybean (Glycine max L. cv. Qihuang 34) growth, surpassing individual stresses without reaching synergistic severity. Physiological analysis revealed additive inhibition, as reflected by a 38.8 % reduction in leaf area, 36.8 % decrease in dry matter accumulation, and a 63.4 % decline in net photosynthetic rate under combined stress conditions, closely aligning with theoretical additive predictions (e.g., observed vs. predicted values: 38.8 % vs. 40.8 % for leaf area; 36.8 % vs. 37.7 % for biomass). Integrated transcriptomic and metabolomic analysis identified hypoxia-induced ATP depletion as a key driver, disrupting Na+/K+ homeostasis (277.9 % increase in Na+/K+ ratio) despite upregulation of key ion transporters (SOS1: +84.3 %, NHX1a: +54.0 % and NHX1b: +68.7 %). Additive activation of flavonoid biosynthesis genes (CHI, CHR) and metabolites (prunin, chalcone 2 '-O-glucoside) mitigated oxidative damage through cumulative metabolite accumulation rather than synergistic interactions. Downregulation of photosynthesis-related genes (e.g., PsbO, PsaA) and ATP synthesis collapse explained energy deficits. Crucially, cutin/suberine biosynthesis emerged as a novel adaptive signature under combined stress. This study provides mechanistic insights into additive salt-waterlogging interactions, identifying targets for stress-resilient soybean breeding.

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