Comparative Transcriptome Analysis of Gene Expression Between Female and Monoecious Spinacia oleracea L.

文献类型： 外文期刊

第一作者： Zhao, Yingjie

作者： Zhao, Yingjie;Zheng, Shaowen;Zhao, Yingjie;Liu, Zhiyuan;She, Hongbing;Xu, Zhaosheng;Zhang, Helong;Qian, Wei

作者机构：

关键词： spinach; monoecious; RNA-seq; differentially expressed genes (DEGs)

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

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年卷期： 2025 年 16 卷 1 期

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收录情况： SCI

摘要： Background: Spinach (Spinacia oleracea L.) is an important leafy vegetable with dioecious and occasional monoecious plants. Monoecious lines are more suitable for hybrid production than dioecious lines due to their extended flowering period. However, genetic research on the sex determination of monoecism remains limited. Methods: In this study, RNA-seq analysis of monoecious and female spinach plants was performed at two distinct flowering stages. In total, we identified 4586 differentially expressed genes (DEGs), which were primarily involved in biological processes such as hormone signaling, cell wall biosynthesis, photosynthesis, and flower development, based on Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results: Among these DEGs, 354 transcription factors, including 27 genes associated with the ABCDE gene, were discovered. Furthermore, a co-expression gene regulatory network was built, identifying nine key genes that play important roles in regulating sex differentiation between female and monoecious plants. Conclusions: Our findings provide crucial molecular insights into the mechanisms of monoecism in spinach and offer a scientific basis for future spinach breeding.

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