Pseudomonas composti isolate from oyster digestive tissue specifically binds with norovirus GII.6 via Psl extracellular polysaccharide
文献类型： 外文期刊
第一作者： Yu, Yongxin
作者： Yu, Yongxin;Han, Feng;Zhang, Xiaoya;Chen, Yunfei;Yu, Mingxia;Wang, Yongjie;Wang, Yongjie;Yu, Yongxin;Wang, Yongjie;Han, Feng;Yu, Mingxia
作者机构：
关键词： Norovirus; Binding; Pseudomonas composti; Exopolysaccharide; Oyster
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）
ISSN： 0168-1605
年卷期： 2023 年 406 卷
页码：
收录情况： SCI
摘要： Oysters are recognized as important vectors for human norovirus transmission in the environment. Whether norovirus binds to bacteria in oyster digestive tissues (ODTs) remains unknown. To shed light on this concern, ODT-54 and ODT-32, positive for histo-blood group antigen (HBGA)-like substances, were isolated from ODTs and identified as Pseudomonas composti and Enterobacter cloacae, respectively. The binding of noroviruses (GII.4 and GII.6 P domains) to bacterial cells (ODT-32 and ODT-54; in situ assay) as well as extracted extracellular polysaccharides (EPSs; in vitro assay) was analyzed by flow cytometry, confocal laser scanning microscopy, ELISA, and gene knock-out mutants. ODT-32 bound to neither GII.4 nor GII.6 P domains, while ODT-54 spe-cifically binds with GII.6 P domain through Psl, an exopolysaccharide encoded by the polysaccharide synthesis locus (psl), identified based on gene annotation, gene transcription, Psl specific staining, and ELISAs. These findings attest that ODT bacteria specifically bind with certain norovirus genotypes in a strain-dependent manner, contributing to a better understanding of the transmission and enrichment of noroviruses in the environment.
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