Dietary Clostridium autoethanogenum protein modulates intestinal absorption, antioxidant status, and immune response in GIFT (Oreochromis niloticus) juveniles

文献类型： 外文期刊

第一作者： Maulu, Sahya

作者： Maulu, Sahya;Ke, Ji;Ren, Mingchun;Ge, Xianping;Huang, Dongyu;Yu, Heng;Liang Hualiang;Ren, Mingchun;Ge, Xianping

作者机构：

关键词： absorption capacity; antioxidant status; bacteria protein; Clostridium autoethanogenum; GIFT juvenile; intestinal health

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2021 年 52 卷 11 期

页码：

收录情况： SCI

摘要： This study investigated the effect of Clostridium autoethanogenum protein (CaP) on intestinal absorption, antioxidant status, and immune response of the genetically improved farmed tilapia (GIFT: Oreochromis niloticus) juveniles. The fish (initial weight 0.7 +/- 0.5 g) were fed with diets containing different levels of CaP: 0% (control), 5%, 10%, 15%, and 20% to partially replace soybean meal. After 8 weeks feeding trial, absorption capacity-related sodium/potassium-ATPase transporter (Na+/K+-ATP) was increased significantly by CaP levels; alkaline phosphatase (AKP) improved significantly by 5% and 10% CaP levels; while gamma-glutamyl transpeptidase (gamma-GT) was not significantly affected compared with the control diet. Malondialdehyde (MDA) content decreased significantly; glutathione (GSH) content and superoxide dismutase (SOD) activity increased significantly; while catalase (CAT) and glutathione peroxidase (GP-x) activities were not significantly affected by dietary CaP compared with the control. The expression of antioxidant-related genes: kelch-like ECH-associated protein 1 (Keap1), nuclear factor erythroid-2-related factor 2 (Nrf2) and glutathione peroxidase (GPx) were significantly down-regulated, up-regulated by 5% and 10%, and not significantly affected by CaP levels compared with the control diet, respectively. The immune-related target of rapamycin (TOR), peptide transporter 1 beta (Pept 1 beta), complementary component 3 (C3) and interferon-gamma (IFN-gamma) were significantly up-regulated by CaP levels; transforming growth factor-beta (TGF-beta) was only significantly up-regulated by 5% and 10% CaP; while interleukin-8 (IL-8) was not significantly affected compared with the control. Therefore, our results show that dietary CaP could enhance the intestinal absorption, antioxidant status, and immune response in GIFT juveniles. However, inclusion levels above 10% could harm the intestinal histology of the fish.

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