Cloning and expression of ophc2, a new organphosphorus hydrolase gene

文献类型： 外文期刊

第一作者： Wu, NF

作者： Wu, NF;Deng, MJ;Liang, GY;Chu, XY;Yao, B;Fan, YL

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关键词： Pseudomonas pseudoalcaligenes;organphosphorus hydrolase;gene cloning and expression

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： The ami no acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence ofophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.

分类号： N1

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