Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration

文献类型: 外文期刊

第一作者: Wu, XM

作者: Wu, XM;Wu, NF;Qian, XZ;Li, RG;Huang, FH;Zhu, L

作者机构:

关键词: Brassica napus;conservation strategies;germplasm;genetic integrity;genomic DNA;mutation;RAPD;seed storage;seed germination;ultra-dry

期刊名称:SEED SCIENCE RESEARCH ( 影响因子:2.25; 五年影响因子:2.5 )

ISSN: 0960-2585

年卷期: 1998 年 8 卷

页码:

收录情况: SCI

摘要: The viability, vigour and genetic variation of rapeseed (Brassica napus L.) plants grown from seeds stored for 11 and 18 years under ultra-dry conditions were compared with growth properties of plants grown from fresher seeds produced by continuous regeneration. There was a inverse relationship between the height and productivity of plants and the duration of seed storage. The strength of this relationship was lessened somewhat by regrowing all the seed lots. The differences in growth and yield that persisted in the F1 generations were believed to result from genetic changes to populations that occurred either during storage or during regeneration. A preliminary PCR-RAPD analysis of plants arising from seeds of different ages confirmed that there were genetic differences among 18-year-old seed (no regeneration), 11-year-old seed (two regeneration cycles) and fresh seed (five regeneration cycles). While the causes for genetic change cannot be ascertained from this experiment, the proportional differences in DNA fragments and the changes in yield are of a magnitude expected for processes such as selection and genetic drift occurring in the regenerated population.

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