Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2

文献类型： 外文期刊

第一作者： Wu, NF

作者： Wu, NF;Deng, MJ;Shi, XY;Liang, GY;Yao, B;Fan, YL

作者机构：

关键词： Pseudomonas pseudoalcaligenes;organphosphorus (OP);organphosphorus hydrolase (OPH);purification;properties

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A bacterium with the capability of degrading organphosphorus, identified as Pseudomonas pseudoaicali-genes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65 deg C and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pEI stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.

分类号： N1

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