Irreversible coumarin based fluorescent probe for selective detection of Cu2+ in living cells

文献类型： 外文期刊

第一作者： Ahmed, Nadeem

作者： Ahmed, Nadeem;Zareen, Wajeeha;Yang, Xiaopeng;Ye, Yong;Ahmed, Nadeem;Zareen, Wajeeha;Yang, Xiaopeng;Ye, Yong;Zhang, Di

作者机构：

关键词： Fluorescent probe; Copper ions; KYSE30 cells; Catalytic hydrolysis; Fluorescence imaging

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.831； 五年影响因子：4.073 ）

ISSN： 1386-1425

年卷期： 2022 年 264 卷

页码：

收录情况： SCI

摘要： Copper ion (Cu2+) is an essential part of the living organisms. Cu2+ ions play a vital role in many biotic processes. An abnormal amount of Cu2+ ions may result in serious diseases. Herein, a novel "fluorescent ON" probe NC-Cu to trace minute levels of Cu2+ ions in presence of various biological active species has been developed. Lysosomal cells targeting group (Morpholine) was added to the probe. The spectral properties of probe NC-Cu were recorded in HEPES buffer (0.01 M, pH = 7.4, comprising 50% CH3CN, lambda(ex) = 430-nm, slit: 5 nm). The synthesized probe NC-Cu work based on copper promoted catalytic hydrolysis of hydrazone and shows remarkable fluorescence enhancement. The reaction of the probe with Cu2+ ions was completed within 20 min. An excellent linear relationship (R-2 = 0.9952) was found and the limit of detection (LOD, according to the 3 sigma/slope) for Cu2+ ions was calculated to be 5.8 mu M. Furthermore, NC-Cu was effectively functional in the living cells (KYSE30 cells) to trace Cu2+ ions. (C) 2021 Elsevier B.V. All rights reserved.

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