文献类型： 外文期刊

第一作者： Liu, Yumeng

作者： Liu, Yumeng;Li, Yujia;Wang, Anbang;Guo, Borui;Tang, Fenling;Li, Jingyang;Liu, Yumeng;Li, Yujia;Wang, Anbang;Guo, Borui;Tang, Fenling;Li, Jingyang;Li, Yujia;Wang, Anbang;Xu, Zhuye;Tang, Fenling;Li, Jingyang;Liu, Yumeng;Guo, Borui;Xu, Zhuye;Wang, Zuo;Li, Chunfang;Chen, Yan

作者机构： Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Sanya 572024, Hainan, Peoples R China;Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Haikou 571101, Hainan, Peoples R China;Chinese Acad Trop Agr Sci, Hainan Banana Hlth Seedling Propagat Engn Res Ctr, Haikou Expt Stn, Haikou 571101, Hainan, Peoples R China;Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China;Hainan Univ, Coll Hort, Haikou 571101, Hainan, Peoples R China;Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China;Yunnan Agr Univ, Collage Trop Crop, Puer 611101, Yunnan, Peoples R China

关键词： Bananas; Cold resistance; Somatic mutations; EMS mutagenesis; L-Hyp pressure selection; Transcriptome analysis; Phenotypic variations

期刊名称：BMC PLANT BIOLOGY （ 2022影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background The cultivation of bananas encounters substantial obstacles, particularly due to the detrimental effects of cold stress on their growth and productivity. A potential remedy that has gained attention is the utilization of ethyl mesylate (EMS)-induced mutagenesis technology, which enables the creation of a genetically varied group of banana mutants. This complex procedure entails subjecting the mutants to further stress screening utilizing L-Hyp in order to identify those exhibiting improved resistance to cold. This study conducted a comprehensive optimization of the screening conditions for EMS mutagenesis and L-Hyp, resulting in the identification of the mutant cm784, which exhibited remarkable cold resistance. Subsequent investigations further elucidated the physiological and transcriptomic responses of cm784 to low-temperature stress. Results EMS mutagenesis had a substantial effect on banana seedlings, resulting in modifications in shoot and root traits, wherein a majority of seedlings exhibited delayed differentiation and limited elongation. Notably, mutant leaves displayed altered biomass composition, with starch content exhibiting the most pronounced variation. The application of L-Hyp pressure selection aided in the identification of cold-resistant mutants among seedling-lethal phenotypes. The mutant cm784 demonstrated enhanced cold resistance, as evidenced by improved survival rates and reduced symptoms of chilling injury. Physiological analyses demonstrated heightened activities of antioxidant enzymes and increased proline production in cm784 when subjected to cold stress. Transcriptome analysis unveiled 946 genes that were differentially expressed in cm784, with a notable enrichment in categories related to 'Carbohydrate transport and metabolism' and 'Secondary metabolites biosynthesis, transport, and catabolism'. Conclusion The present findings provide insights into the molecular mechanisms that contribute to the heightened cold resistance observed in banana mutants. These mechanisms encompass enhanced carbohydrate metabolism and secondary metabolite biosynthesis, thereby emphasizing the adaptive strategies employed to mitigate the detrimental effects induced by cold stress.

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