Identification of endoplasmic-reticulum-associated proteins involved in Bombyx mori nucleopolyhedrovirus entry by RNA-seq analysis

文献类型： 外文期刊

第一作者： Hao, Bifang

作者： Hao, Bifang;Li, Chenya;Amanze, Charles;Huang, Jinshan;Hao, Bifang;Li, Chenya;Amanze, Charles;Huang, Jinshan

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.685； 五年影响因子：2.506 ）

ISSN： 0304-8608

年卷期： 2022 年 167 卷 4 期

页码：

收录情况： SCI

摘要： Membrane fusion is a key step in enveloped virus infection, releasing the viral genome into the cytoplasm to initiate infection. Bombyx mori nucleopolyhedrovirus (BmNPV) is an enveloped DNA virus that mainly infects silkworms. Information about membrane fusion of BmNPV with host cells is still limited. In this study, BmN cells were pretreated with ??ammonium chloride??, and infection with BmNPV was allowed to occur naturally through endocytosis or artificially through low-pH-induced fusion with the plasma membrane, after which the cells were subjected to RNA-seq. The results indicated that a few endoplasmic reticulum-associated proteins (ERAPs) were among the common upregulated DEGs, including BiP, CRT, and HSP90, and this upregulation was confirmed by q-PCR. Knockdown of BiP, CRT, and HSP90 expression by siRNA resulted in significant inhibition of BmNPV infection. This study suggests that ERAPs may be involved in the BmNPV membrane fusion process during infection.

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