Hepatoprotective and antioxidant effects of dietary Glycyrrhiza polysaccharide against TCDD-induced hepatic injury and RT-PCR quantification of AHR2, ARNT2, CYP1A mRNA in Jian Carp (Cyprinus carpio var. Jian)

文献类型： 外文期刊

第一作者： Du, Jinliang

作者： Du, Jinliang;Cao, Liping;Jia, Rui;Yin, Guojun;Du, Jinliang;Cao, Liping;Jia, Rui;Yin, Guojun;Yin, Guojun

作者机构：

关键词： Aryl hydrocarbon receptor 2 (AHR2); Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2); Cytochrome P4501A (CYP1A); Cyprinus carpio var. Jian; Glycyrrhiza polysaccharide; 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)

期刊名称：JOURNAL OF ENVIRONMENTAL SCIENCES （ 影响因子：5.565； 五年影响因子：5.066 ）

ISSN： 1001-0742

年卷期： 2017 年 51 卷

页码：

收录情况： SCI

摘要： To evaluate the protective effects of Glycyrrhiza polysaccharide (GPS) against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced hepatotoxicity in Jian carp, the fish were fed diets containing GPS at doses of 0.1, 0.5 and 1.0 g/kg for 60 days before an intraperitoneal injection of 0.6 mu g/kg TCDD at a volume of 0.05 mL/10 g body weight. At 72 hr post-injection, blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices. The results showed that increases in alanine aminotransferase (GPT), aspartate aminotransferase (GOT), lactate dehydrogenase (LDH), and alkaline phosphatase (AKP) in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0 g/kg GPS. Following the 1.0 g/kg GPS pre-treatment, total protein (TP), albumin (Alb), catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activities in liver tissue increased significantly, malondialdehyde (MDA) formation (P < 0.05 or P < 0.01) was significantly inhibited, and the expression of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor 2 (AHR2) and aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) mRNA (P < 0.05) was significantly enhanced. Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining, and the results showed that histological changes were obviously reduced by 0.5 and 1.0 g/kg GPS. GPS significantly reduced liver tissue damage caused by TCDD. Overall, these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD, and supported the use of GPS (1.0 g/kg) as a hepatoprotective and antioxidant agent in fish. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序