Molecular characterization and expression of complement factor I in Pelteobagrus vachellii during Aeromonas hydrophila infection

文献类型： 外文期刊

第一作者： Wen, Zhengyong

作者： Wen, Zhengyong;Yuan, Dengyue;Gong, Quan

作者机构：

关键词： Pelteobagrus vachellii; Complement factor I; Immunity; cDNA

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2018 年 82 卷

页码：

收录情况： SCI

摘要： Complement factor I (CFI) is a novel regulatory serine protease that plays an important role in resistance to pathogen infection. In this study, the CFI gene of Pelteobagrus vachellii (Pv-CFI) was sequenced and characterized. The full-length cDNA of 2320 bp includes a 155 bp 5'-untranslated region (UTR), a 164 bp 3'-UTR, and a 2001 bp open reading frame (ORF) encoding a 667 amino acids. Multiple sequence alignment revealed five highly conserved domains with a typical modular architecture and identical active sites in vertebrates, indicating a conserved function. Pv-CFI mRNA was constitutively expressed in all examined tissues and most abundant in liver. During infection with Aeromonas hydrophila, Pv-CFI mRNA expression was significantly up-regulated in liver at 3-24 h, spleen at 3-48 h and head kidney at 3-48 h. The results suggest Pv-CFI plays an important role in resistance to pathogenic bacteria in P. vachellii. (C) 2018 Elsevier Ltd. All rights reserved.

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