Molecular characterization and expression of toll-like receptor 5 genes from Pelteobagrus vachellii
文献类型： 外文期刊
第一作者： Wen, Zhengyong
作者： Wen, Zhengyong;Yuan, Dengyue;Shao, Ting;Li, Huatao;Gong, Quan
作者机构：
关键词： Toll-like receptor 5; Pelteobagrus vachellii; Aeromonas hydrophila; Toll-like receptor pathway; Pathogen challenge; Innate immunity
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2018 年 75 卷
页码：
收录情况： SCI
摘要： Toll-like receptor 5 (TLR5) is an important pathogen recognition receptor (PRR) that recognizes the flagellin protein of pathogenic bacteria and plays a fundamental role in activating the innate immune response. In this study, full-length pvTLRSm (membrane) and pvTLRSs (soluble) genes were cloned from darkbarbel catfish Pelteobagrus vachellii, and their expression and that of downstream genes were analyzed following exposure to the Aeromonas hydrophila pathogen. The 3009 bp pvTLRSm cDNA includes a 2652 bp open reading frame (ORF) encoding 884 amino acids. The 2422 by pvTLR5s cDNA includes a 1944 bp ORF encoding a predicted protein of 648 amino acids. The genes are most closely related to TLR5m (75%) and TLR5s (69%) from Ictalurus punctatus, respectively, and both have a typical TLR structure. Both genes were constitutively expressed in all examined tissues, and most abundantly in the head kidney and spleen. Following pathogen challenge, pvTLRSm and pvTLRSs expression was increased significantly (P < 0.05) and peaked at 24 and 12 h post-exposure in the liver, 24 and 12 h in the head kidney, and 48 and 24 h in the spleen, respectively. The downstream genes interleukin-1 beta (IL-1 beta), IL-12 and tumor necrosis factor-alpha (TNF-alpha) were significantly up-regulated following pathogen exposure in spleen, and the NF-kB inhibitor (I kappa B) was down-regulated. These findings indicated that pvTLR5 may play an important role in the immune responses to A. hydrophila. These results provide new insight to elucidate the immune signalling pathways of fish TLR.
分类号：
- 相关文献
作者其他论文 更多>>
-
Histological, ultrastructural, and multi-omic integration analysis on the response mechanisms of Acipenser dabryanus to hypoxia and reoxygenation
作者：Chen, Yeyu;Wu, Xiaoyun;Li, Pengcheng;Lai, Jiansheng;Ni, Luyun;Liu, Zhao;Song, Mingjiang;Li, Feiyang;Gong, Quan;Chen, Yeyu;Wu, Xiaoyun;Li, Pengcheng;Lai, Jiansheng;Ni, Luyun;Liu, Zhao;Song, Mingjiang;Li, Feiyang;Gong, Quan
关键词：Hypoxia; Reoxygenation; Acipenser dabryanus; Multi-omics
-
Multiplex one-step RT-qPCR assays for simultaneous detection of BRV, BCoV, Escherichia coli K99+ and Cryptosporidium parvum
作者：Zhao, Xinru;Li, Min;Yang, Yingying;Wang, Yidan;Zheng, Xiaoru;Li, Huatao;Fu, Kaiqiang;Cao, Zhi;Yin, Dehua;Gao, Haihui
关键词：BRV; BCoV;
E. coli K99(+);C. parvum ; multiplexTaq Man real-time PCR -
Exploring the adsorption sites and mechanism of biochar towards tetracycline using machine learning
作者：Xian, Bo;Li, Qingzhi;Zhao, Huayi;Gong, Quan;Xian, Bo;Li, Qingzhi;Zhao, Huayi;Gong, Quan
关键词：Machine learning; Biochar; Tetracycline; Adsorption mechanism; Feature selection
-
The Effects of Dietary Supplementation with 25-Hydroxyvitamin D3 on the Antioxidant Capacity and Inflammatory Responses of Pelteobagrus fulvidraco
作者：Liu, Yi;Xie, Jiang;Shi, Qingchao;Qin, Chuanjie;Liu, Yi;Xie, Jiang;Shi, Qingchao;Qin, Chuanjie;Liu, Yi;Xie, Jiang;Shi, Qingchao;Qin, Chuanjie;Gong, Quan
关键词：metabolome; transcriptome; yellow catfish; 25(OH)D3; antioxidant; inflammatory
-
5 mC modification of steroid hormone biosynthesis-related genes orchestrates feminization of channel catfish induced by high-temperature
作者：Xu, Siqi;Xie, Bingjie;Liu, Hongyan;Liu, Ju;Wang, Minghua;Zhong, Liqiang;Chen, Xiaohui;Zhang, Shiyong;Xu, Siqi;Liu, Hongyan;Wang, Minghua;Zhong, Liqiang;Chen, Xiaohui;Zhang, Shiyong;Chen, Xiaohui;Zhang, Shiyong;Xie, Bingjie;Chen, Xiaohui;Zhou, Jian;Zhang, Lu;Wen, Zhengyong
关键词：Temperature; Sex reversal; Methylation; srd5a2; Channel catfish
-
Comparison of muscle structure and transcriptome analysis reveals the mechanism of growth variation in Yangtze sturgeon (Acipenser dabryanus)
作者：Li, Qingzhi;Chen, Yeyu;Wu, Xiaoyun;Liu, Ya;Lai, Jiansheng;Song, Mingjiang;Li, Feiyang;Li, Pengcheng;He, Bin;Wang, Bin;Yan, Tao;Gong, Quan;Li, Qingzhi;Wang, Bin;Gong, Quan;Wang, Jun;Wang, Jun
关键词：Acipenser dabryanus; Growth difference; GH/IGF axis; Myogenic regulatory factor; Cell cycle
-
Genome sequencing and transcriptome analysis provide an insight into the hypoxia resistance of Channa asiatica
作者：Zhou, Chaowei;Li, Junting;Duan, Yuting;Fu, Suxing;Li, Hejiao;Zhou, Yinhua;Zhou, Xinghua;Lei, Luo;Yuan, Dengyue;Zhou, Chaowei;Li, Junting;Duan, Yuting;Fu, Suxing;Li, Hejiao;Zhou, Yinhua;Gao, He;Zhou, Xinghua;Liu, Haiping;Lei, Luo;Yuan, Dengyue;Gao, He;Liu, Haiping;Chen, Jie
关键词：Channa asiatica; de novo genome assembly; Transcriptome analysis; Gene family expansion; Hypoxia tolerance
