Effects of CeCl3 and LaCl3 on callus and root induction and the physical response of tobacco tissue culture

文献类型： 外文期刊

第一作者： Song, Guicheng

作者： Song, Guicheng;Zhang, Pingping;Shi, Gaoling;Wang, Huadun;Ma, Hongxiang

作者机构：

关键词： Cerium chloride; Lanthanum chloride; Callus induction; Tissue culture; POD expression; Stomata

期刊名称：JOURNAL OF RARE EARTHS （ 影响因子：3.712； 五年影响因子：3.326 ）

ISSN： 1002-0721

年卷期： 2018 年 36 卷 4 期

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收录情况： SCI

摘要： La3+ and Ce3+ have positive effects on plant growth and production. Although it is well known that rare earth elements promote cell growth, the biological effects of La3+ and Ce3+ on callus, shoot and root induction in tobacco are still unclear. The relationships among callus induction, rooting, enzyme activities and stomatal characteristics in tobacco are unknown. The objectives of this study were to identify the relationships between the induction of calluses, shoots, roots, stomata and enzyme activities. The induction percentages of calluses, buds, roots were recorded at 5, 10, 15, 20 and 25 days after La3+ and Ce3+ treatments. Peroxidase isoenzyme activity was determined by electrophoresis. The characteristics of the stomata were observed under an optical microscope. Our results show that low concentrations of Ce3+ (< 15 mg/L) result in increases in the induction percentages of calluses, buds and roots, but La3+ (> 5 mg/L) inhibits the induction of calluses, buds and roots. There are more peroxidase isoenzyme bands in Ce3+ treatments than in La3+ treatments. This is consistent with the induction percentages of calluses, buds and roots in Ce3+ and La3+ treatments. High enzyme activities may promote the induction of calluses, buds and roots. The stomata area and stomata number of leaves are significantly different between La3+ treatments and Ce3+ treatments. La3+ improves the stomata area and number. Based on these results, we speculate that La3+ may promote the development of the photosynthetic system. Ce3+ may promote tobacco growth and rooting by improving enzyme activities. (c) 2018 Published by Elsevier B.V. on behalf of Chinese Society of Rare Earths.

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