GC-MS analysis of the ruminal metabolome response to thiamine supplementation during high grain feeding in dairy cows

文献类型： 外文期刊

第一作者： Xue, Fuguang

作者： Xue, Fuguang;Pan, Xiaohua;Xiong, Benhai;Xue, Fuguang;Guo, Yuming;Jiang, Linshu

作者机构：

关键词： Thiamine; SARA; Metabolomics; Pyruvate metabolism; Dairy cows

期刊名称：METABOLOMICS （ 影响因子：4.29； 五年影响因子：4.214 ）

ISSN： 1573-3882

年卷期： 2018 年 14 卷 5 期

页码：

收录情况： SCI

摘要： Introduction Thiamine is known to attenuate high-concentrate diet induced subacute ruminal acidosis (SARA) in dairy cows, however, the underlying mechanisms remain unclear. Objectives The major objective of this study was to investigate the metabolic mechanisms of thiamine supplementation on high-concentrate diet induced SARA. Methods Six multiparous, rumen-fistulated Holstein cows were used in a replicated 3 x 3 Latin square design. The treatments included a control diet (CON; 20% starch, dry matter basis), a SARA-inducing diet (SAID; 33.2% starch, dry matter basis) and SARA-inducing diet supplemented with 180 mg of thiamine/kg of dry matter intake (SAID + T). On d21 of each period, ruminal fluid samples were collected at 3 h post feeding, and GC/MS was used to analyze rumen fluid samples. Results PCA and OPLS-DA analysis demonstrated that the ruminal metabolite profile were different in three treatments. Compared with CON treatment, SAID feeding significantly decreased rumen pH, acetate, succinic acid, increased propionate, pyruvate, lactate, glycine and biogenic amines including spermidine and putrescine. Thiamine supplementation significantly decreased rumen content of propionate, pyruvate, lactate, glycine and spermidine; increase rumen pH, acetate and some medium-chain fatty acids. The enrichment analysis of different metabolites indicated that thiamine supplementation mainly affected carbohydrates, amino acids, pyruvate and thiamine metabolism compared with SAID treatment. Conclusions These findings revealed that thiamine supplementation could attenuate high-concentrate diet induced SARA by increasing pyruvate formate-lyase activity to promote pyruvate to generate acetyl-CoA and inhibit lactate generation. Besides, thiamine reduced biogenic amines to alleviate ruminal epithelial inflammatory response.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序