Effect of Coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes

文献类型： 外文期刊

第一作者： Fan, Qiuxia

作者： Fan, Qiuxia;Zhang, Yanyan;Wu, Qian;Shi, Chao;Zhang, Chunling;Xia, Xiaodong;Wang, Xin;Yang, Hua

作者机构：

关键词： Listeria monocytogenes; Biofilm; Coenzyme Q0; Gene expression

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 90 卷

页码：

收录情况： SCI

摘要： Listeria monocytogenes (L. monocytogenes) is a highly pathogenic microorganism that could cause food borne diseases and a series of infections. Coenzyme Q0 (CoQ0) has been reported as antineoplastic and anti-inflammatory compound, but its antimicrobial and antibiofllm activity against L. monocytogenes has scarcely been studied. In this study, antimicrobial activity of CoQ0 against L. monocytogenes was determined. The efficacy of CoQ0 in inhibiting L. monocytogenes biofilm formation on three surfaces (polystyrene, stainless steel, and glass), inactivating and removing formed biofilm, as well as reducing adhesion to and invasion of human colon adenocarcinoma (Caco-2) cells by L. monocytogenes were investigated. Moreover, real time quantitative PCR (RT-qPCR) was carried out to evaluate the effect of CoQ0 on expression of genes involved in biofilm formation, adhesion, and invasion. Minimum inhibitory concentrations (MICs) of CoQ0 against L. monocytogenes strains ranged from 25 to 50 mu g/ml. CoQ0 was effective in preventing biofilm development on three different surfaces, as confirmed by scanning electron microscope (SEM) observation. In addition, CoQ0 inactivated and removed mature biofilm formed by L. monocytogenes, reduced adhesion to and invasion of Caco-2 cells, and down-regulated transcription levels of related genes. These findings suggest that CoQ0 has the potential to be a new strategy to control L. monocytogenes. (C) 2018 Elsevier Ltd. All rights reserved.

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