The D5 region of the intelectin domain is a new type of carbohydrate recognition domain in the intelectin gene family

文献类型： 外文期刊

第一作者： Yan, Jie

作者： Yan, Jie;Chen, Lei;Feng, Lijun;Chen, Lei;Liu, Zhuang;Chen, Yonglin;Sun, Ying;Han, Jia

作者机构：

关键词： Intelectin; CRD; Polysaccharide binding; Comparative genomics

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2018 年 85 卷

页码：

收录情况： SCI

摘要： Intelectin is a recently characterized soluble galactofuranose-binding lectin that exists in species ranging from amphioxus to human. Interestingly, intelectin does not contain a canonical carbohydrate-recognition domain (CRD). Therefore, we designed serial deletions of intelectin in the Chinese amphioxus (Branchiostoma belcheri tsingtauense, AmphilTLN71469) in order to identify functional regions required for carbohydrate binding. Our results revealed that Domain 5 (aa 203-302) was able to bind lipopolysaccarides (LPS) or peptidoglycan (PGN) and agglutinate bacteria as efficiently as the full-length protein. Three dimensional (3D) atomic models of Domain 5 were generated by ab initio based program QUARK and by Iterative Threading Assembly Refinement (I-TASSER) programs, in which four amino acids mediating calcium-binding (G54-G55-G56-E91) were identified by hemagglutination assay. Furthermore, a striking functional conservation of Domain 5 was detected in zebrafish intelectin 1. Taken together, our findings identified for the first time a new CRD domain in intelectin, thereby providing new knowledge leading to a better understanding of pathogen-host interactions. (C) 2018 Elsevier Ltd. All rights reserved.

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