Dulplex analysis of mercury and silver ions using a label-free fluorescent aptasensor

文献类型： 外文期刊

第一作者： Zhang, Juan

作者： Zhang, Juan;Huang, Yafei;Yan, Jiao;Zhu, Chao;Chen, Ailiang;Zhang, Juan;Huang, Yafei;Yan, Jiao;Zhu, Chao;Chen, Ailiang;Huang, Yafei;Yan, Jiao;Zhang, Chenghui

作者机构：

关键词： Aptamer; PicoGreen; mercury; silver; label-free; aptasensor

期刊名称：INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY （ 影响因子：2.826； 五年影响因子：2.215 ）

ISSN： 0306-7319

年卷期： 2018 年 98 卷 4 期

页码：

收录情况： SCI

摘要： Label-free Hg2+ aptamer was used as a sensing element and the PicoGreen dye was specific to ultra-sensitive double-stranded DNA (dsDNA), which achieved novel fluorescence assay for detection of both mercury and silver ions. In this aptasensor, Hg2+ bound to thymidine (T) to form T-Hg2+-T base pairs and Ag+ specifically interacted with C-C mismatches to produce C-Ag+-C base pairs. The conformation changes prevented the aptamer from binding to its complementary sequences to form dsDNA and caused a fluorescence intensity decrease with PicoGreen. The change in the fluorescence intensity made it possible to detect both Hg2+ and Ag+ in a dose-dependent manner. The sensing system could detect as low as 5x10(-8)mol/L of Hg2+ and 9.3x10(-10)mol/L of Ag+. The fluorescent intensity changes in the system were specific for Hg2+ and Ag+, making this simple and cost-effective method extremely valuable in its future applications in monitoring Hg2+ and Ag+ pollution in environmental analysis.

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