Downregulation of aldose reductase is responsible for developmental abnormalities of the silkworm purple quail-like mutant (q-l(p))

文献类型： 外文期刊

第一作者： Wang, Pingyang

作者： Wang, Pingyang;Bi, Simin;Wei, Weiyang;Qiu, Zhiyong;Xia, Dingguo;Shen, Xingjia;Zhao, Qiaoling

作者机构：

关键词： Aldose reductase; Sorbitol; Purple quail-like; Bombyx mori; Diabetic complications

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 665 卷

页码：

收录情况： SCI

摘要： Aldose reductase (AR) is a rate-limiting enzyme in the polyol pathway and is also the key enzyme involved in diabetic complications. The silkworm purple quail-like mutant (q-l(p)) exhibits pigmented dots on its epidermis. The q-l(p) mutant also shows developmental abnormalities and decreased vitality. In this study, fat bodies from the q-l(p) mutant and the wildtype 932VR strain were subjected to two-dimensional gel electrophoresis (2-DE) analysis, and the Bombyx mori AR (BmAR) protein was found to be significantly downregulated in the q-l(p) mutant. The expression of BmAR at the mRNA level was also significantly downregulated, as verified through quantitative reverse transcription PCR (qRT PCR). Knockdown of the expression of BmAR via RNAi resulted in a reduction of silkworm weight. The sorbitol level in q-l(p) was significantly lower than in the wildtype. These results suggested that the B,nAR gene is closely related to the development of the q-l(p) mutant. Investigation of the cause of BmAR downregulation in the q-l(p) mutant could contribute to revealing the function of AR in insects and offers a new method of identifying AR inhibitors for the treatment of diabetic complications.

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