Transcriptome characterization and differential expression analysis of disease-responsive genes in alfalfa leaves infected by Pseudopeziza medicaginis

文献类型： 外文期刊

第一作者： Li, Yue

作者： Li, Yue;Wang, Yu;Yuan, Qinghua;Huang, Haiyan

作者机构：

关键词： Alfalfa; Common leaf spot; Comparative transcriptome; Disease-responsive genes

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2018 年 214 卷 7 期

页码：

收录情况： SCI

摘要： Common leaf spot (CLS) which caused by Pseudopeziza medicaginis is a serious foliar disease in alfalfa. Here, we report a differential expression model of transcriptome between leaves from a CLS-resistant line (R-I vs. R-ck) and a CLS-susceptible line (S-I vs. S-ck) after inoculation with P. medicaginis using RNA-seq technology on genome-wide. De novo assembling of clean reads generated 83,625 transcripts with 35,061 unigenes, of which, 85% were found to be able to match the reference genes. 3304 differentially expressed genes (DEGs) were detected in R-I versus R-ck library while 4276 DEGs were found in S-I versus S-ck library. Gene ontology function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these DEGs were performed in this work, and the results showed that some categories were significantly enriched in R-I versus R-ck library and not in S-I versus S-ck library including response to stimulus, response to stress, oxidation-reduction process, and oxidoreductase activity while KEGG pathway analysis suggested that plant-pathogen interaction was one of the main biological pathways in which DEGs in R-I versus R-ck library were enriched, but not that for DEGs in S-I versus S-ck library. Furthermore, about only 20 DEGs showed obviously being up-regulated in R-I versus R-CK library and being down-regulated or not being detected in S-I versus S-CK library and the analysis on the signal transduction pathways related to these DEGs indicated that both SA-mediated signal transduction pathway and ET-mediated signal transduction pathway were related to the CLS resistance of alfalfa. In this work, we comprehensively and systematically characterized the molecular basis of alfalfa in response to P. medicaginis for the first time. The information resulted from this study is useful for future investigating the mechanism of CLS resistance on molecular level in alfalfa.

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