Molecular cloning, identification of GSTs family in sunflower and their regulatory roles in biotic and abiotic stress

文献类型： 外文期刊

第一作者： Ma, Ligong

作者： Ma, Ligong;Ma, Ligong;Zhang, Yunhua;Meng, Qinglin;Shi, Fengmei;Liu, Jia;Li, Yichu

作者机构：

关键词： Glutathione-S-transferase; Sunflower; HaGSTs; Biotic stress; Abiotic stress; Oxidative stress

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2018 年 34 卷 8 期

页码：

收录情况： SCI

摘要： Glutathione-S-transferase (GST) genes exist widely in plants and play major role in metabolic detoxification of exogenous chemical substances and oxidative stress. In this study, 14 sunflower GST genes (HaGSTs) were identified based on the sunflower transcriptome database that we had constructed. Full-length cDNA of 14 HaGTSs were isolated from total RNA by reverse transcription PCR (RT-PCR). Sunflower was received biotic stress (Sclerotinia sclerotiorum) and abiotic stress (NaCl, low-temperature, drought and wound). GST activity was measured by using the universal substrate. The results showed that most of the HaGSTs were up-regulated after NaCl and PEG6000-induced stresses, while a few HaGSTs were up-regulated after S. sclerotiorum, hypothermia and wound-induced stressed, and there was correlation between the changes of GST activity and the expression of HaGSTs, indicating that HaGSTs may play regulatory role in the biotic and abiotic stress responses. 14 HaGSTs from sunflower were identified, and the expression of HaGSTs were tissue-specific and played regulatory roles in both stress and abiotic stress.

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