Identification and application of self-binding zipper-like sequences in SARS-CoV spike protein

文献类型： 外文期刊

第一作者： Zhang, Si Min

作者： Zhang, Si Min;Neo, Tuan Ling;Lu, Yanning;Liu, Ding Xiang;Tam, James P.;Zhang, Si Min;Vahlne, Anders;Liao, Ying

作者机构：

关键词： Steric beta-zipper; SARS-CoV spike protein; Class I viral fusion glycoprotein; self-binding peptides; viral detection

期刊名称：INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY （ 影响因子：5.085； 五年影响因子：4.407 ）

ISSN： 1357-2725

年卷期： 2018 年 101 卷

页码：

收录情况： SCI

摘要： Self-binding peptides containing zipper-like sequences, such as the Leu/Ile zipper sequence within the coiled coil regions of proteins and the cross-beta spine stenc zippers within the amyloid-like fibrils, could bind to the protein- of-origin through homophilic sequence-specific zipper motifs. These self-binding sequences represent opportu nities for the development of biochemical tools and/or therapeutics. Here, we report on the identification of a putative self-binding beta-zipper-forming peptide within the severe acute respiratory syndrome associated coronavirus spike (S) protein and its application in viral detection. Peptide array scanning of overlapping peptides covering the entire length of S protein identified 34 putative self-binding peptides of six clusters, five of which contained octapeptide core consensus sequences. The Cluster I consensus octapeptide sequence GINITNFR was predicted by the Eisenberg's 3D profile method to have high amyloid-like fibrillation potential through stenc beta-zipper formation. Peptide C6 containing the Cluster I consensus sequence was shown to oligomenze and form amyloid-like fibrils. Taking advantage of this, C6 was further applied to detect the S protein expression in vitro by fluorescence staining. Meanwhile, the coiled-coil-forming Leu/Ile heptad repeat sequences within the S protein were under-represented during peptide array scanning, in agreement with that long peptide lengths were required to attain high helix-mediated interaction avidity. The data suggest that short beta-zipper-like self-binding peptides within the S protein could be identified through combining the peptide scanning and predictive methods, and could be exploited as biochemical detection reagents for viral infection.

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