Transcriptome profiling of immune-responsive genes in the intestine of Cynoglossus semilaevis Gunther challenged with Shewanella algae

文献类型： 外文期刊

第一作者： Han, Zhuoran

作者： Han, Zhuoran;Sun, Jingfeng;Lv, Aijun;Sung, Yeong Yik;Sun, Xueliang;Hu, Xiucai;Xing, Kezhi;Xian, Jian-An;Sung, Yeong Yik

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关键词： Cynoglossus semilaevis; Shewanella algae; Intestine; Transcriptone; High-throughput RNA sequencing

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 80 卷

页码：

收录情况： SCI

摘要： To better understand gene expression in the intestine after Shewanella algae infection and provide insights into its immune roles in the tongue sole, Cynoglossus semilaevis, sequencing-based high-throughput RNA analysis (RNA-Seq) for the intestines between the control group and 12 h post-injection group was performed. After assembly, there was an average of 23,957,159 raw sequencing reads, and 23,943,491 clean reads were obtained after filtering out low-quality reads. Then, 383 differentially expressed genes (DEGs) in the intestines in response to S. algae infection were identified. Subsequently, gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the DEGs were conducted to further explore their functions. Among all of the pathways involved, sixteen pathways were related to the immune system, among which the complement and coagulation cascades pathway was the most prominent for immunity-related DEGs, followed by the leukocyte transendothelial migration pathway. Furthermore, the expression levels of twelve selected DEGs in the immune-related pathways were identified by quantitative real-time polymerase chain reaction, substantiating the reliability and reproducibility of the RNA-Seq results. In summary, this study represents an important genomic resource for understanding the potential immune role of the tongue sole intestine from the perspective of gene expression.

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