Optimization of Dynamic Microwave-Assisted Extraction of Dihydromyricetin from Ampelopsis grossedentata using Response Surface Methodology
文献类型： 外文期刊
第一作者： Li, Wei
作者： Li, Wei;Chen, Yuzhen;Xu, Xiang;Zheng, Cheng;Zhao, Jian
作者机构：
关键词： Dihydromyricetin; Ampelopsis grossedentata; Dynamic microwave-assisted extraction; Response surface methodology
期刊名称：JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN （ 影响因子：0.536； 五年影响因子：0.585 ）
ISSN： 0253-5106
年卷期： 2018 年 40 卷 6 期
页码：
收录情况： SCI
摘要： Dihydromyricetin is a natural flavonoid and principal component of the Chinese herbal tea, Ampelopsis grossedentata, with numerous health-promoting bioactivities. Response surface methodology (RSM) was employed to optimize the extraction of dihydromyricetin from Ampelopsis grossedentata leaves using dynamic microwave assisted multi-stage countercurrent extraction. The influence of temperature, pH, time and solvent/material ratio on the yield of extraction was investigated. It was found that the extraction data were sufficiently fitted into a second-order polynomial model (R-2 = 0.9992). The extraction parameters of temperature, pH and solvent/material ratio, the quadrics of time, temperature and pH, and the interaction between each two of the four extraction parameters had a significant effect on the yield. The optimal conditions for extracting dihydromyricetin were predicted to be: temperature, 96.8 degrees C; time, 8.8 min; solvent/material ratio, 26.4/1 and pH, 5.3. Under those conditions, the predicted yield was 92.3% of dihydromyricetin in the leaves.
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