Evaluation of reference genes for RT-qPCR analysis in wild and cultivated Cannabis

文献类型： 外文期刊

第一作者： Guo, Rong

作者： Guo, Rong;Guo, Hongyan;Zhang, Qingying;Guo, Mengbi;Xu, Yanping;Lv, Pin;Chen, Xuan;Yang, Ming;Zeng, Min

作者机构：

关键词： Cannabis; wild; cultivated; reference gene; gene expression

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

ISSN： 0916-8451

年卷期： 2018 年 82 卷 11 期

页码：

收录情况： SCI

摘要： RT-qPCR has been widely used for gene expression analysis in recent years. The accuracy of this technique largely depends on the selection of suitable reference genes. In order to facilitate gene expression analysis in wild and cultivated Cannabis, the expression stability of seven candidate reference genes (ACT2, 18S rRNA, GAPDH, UBQ, TUB, PP2A and EF1 alpha) were assessed in leaves samples of different development stages and different organs of both wild and cultivated Cannabis in the present study. Their expression stabilities were evaluated through three software packages (GeNorm, Normfinder and Bestkeeper). Results showed that UBQ and EF1 alpha were the highly ranked genes in different leaves samples, and PP2A was the most stable reference gene in different organs, while GAPDH was the least stable one. And the validation of the reference genes selected was further confirmed by the expression patterns of MDS and OLS.

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