iTRAQ-based proteomic analysis reveals changes in response to UV-B treatment in soybean sprouts

文献类型： 外文期刊

第一作者： Jiao, Caifeng

作者： Jiao, Caifeng;Gu, Zhenxin

作者机构：

关键词： UV-B; iTRAQ; GABA; Inositol phosphate metabolism; ABA; Soybean sprouts

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 275 卷

页码：

收录情况： SCI

摘要： It has been shown that 15 mu W.cm(-2) UV-B radiation has the most pronounced effects on gamma-aminobutiric acid (GABA), inositol 1,4,5-trisphosphate (IP3) and abscisic acid (ABA) accumulation in 4-day-old soybean sprouts. Nevertheless, its mechanism of action, from the perspective of protein expression, remains largely unknown. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) were employed to investigate UV-B treatment-induced proteomic changes in soybean sprouts. Results showed that UV-B treatment effectively regulated proteins involved in GABA biosynthesis, such as glutamate synthase, glutamate decarboxylase (GAD), methionine synthetase, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, aminoaldehyde dehydrogenase (AMADH) and inositol phosphate metabolism pathways, including phosphoinositide phospholipase C (PI-PLC), purple acid phosphatase (PAP) and inositol polyphosphate 5-phosphatase. In addition, proteins involved in ABA biosynthesis and signal transduction, such as 9-cis-epoxycarotenoid dioxygenase (NCED), abscisic-aldehyde oxidase (AO), SNFl-related protein kinase (SnRK), protein phosphatase 2C (PP2C), guanine nucleotide-binding protein and calreticulin-3, were also modulated under UV-B treatment.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序