Polyaspartic Acid Improves Maize (Zea mays L.) Seedling Nitrogen Assimilation Mainly by Enhancing Nitrate Reductase Activity

文献类型： 外文期刊

第一作者： Wang, Qingyan

作者： Wang, Qingyan;Tang, Huihui;Li, Guangyan;Dong, Hui;Dong, Xuerui;Xu, Yanli;Dong, Zhiqiang

作者机构：

关键词： polyaspartic acid; nitrate reductase; nitrogen metabolism; enzymatic activity; gene expression; protein accumulation

期刊名称：AGRONOMY-BASEL （ 影响因子：3.417； 五年影响因子：3.64 ）

ISSN： 2073-4395

年卷期： 2018 年 8 卷 9 期

页码：

收录情况： SCI

摘要： Improvement of nitrogen use efficiency is of great importance in maize (Zeamays L.) production. In the present study, an eco-friendly growth substance, polyaspartic acid (PASP), was applied to maize seedlings grown with different nitrate (NO3-) doses by foliar spraying, aimed at evaluating its effects on maize nitrogen assimilation at both the physiological and molecular level. The results showed that PASP promoted biomass and nitrogen accumulation in maize seedlings, especially under low NO3- doses. Among different NO3- conditions, the most noticeable increase in plant biomass by PASP addition was observed in seedlings grown with 1 mmol L-1 NO3-, which was a little less than the optimum concentration (2 mmol L-1) for plant growth. Furthermore, the total nitrogen accumulation increased greatly with additions of PASP to plants grown under suboptimal NO3- conditions. The promotion of nitrogen assimilation was mostly due to the increase of nitrate reductase (NR) activities. The NR activities in seedlings grown under low NO3- doses (0.5 and 1.0 mmol L-1) were extremely increased by PASP, while the activities of glutamine synthetase (GS), aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT) were slightly changed. Moreover, the regulation of PASP on NR activity was most probably due to the promotion of the protein accumulation rather than gene expression. Accumulation of NR protein was similarly affected as NR activity, which was markedly increased by PASP treatment. In conclusion, the present study provides insights into the promotion by PASP of nitrogen assimilation and identifies candidate regulatory enzymatic mechanisms, which warrant further investigation with the use of PASP in promoting nitrogen utilization in crops.

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