p15 encoded by Garlic virus X is a pathogenicity factor and RNA silencing suppressor
文献类型: 外文期刊
第一作者: Zhang, Tianhao
作者: Zhang, Tianhao;Zhao, Xing;Yang, Xue;Chen, Ying;Wu, Yuanhua;Jiang, Liangliang;Lu, Yuwen;Peng, Jiejun;Zheng, Hongying;Chen, Jianping;Yan, Fei;Jiang, Liangliang;Lu, Yuwen;Peng, Jiejun;Zheng, Hongying;Chen, Jianping;Yan, Fei;Jiang, Liangliang;Song, Xijiao;MacFarlane, Stuart
作者机构:
关键词: Allexivirus; Garlic virus X; pathogenicity factor; RNA silencing suppressor; cysteine-rich protein
期刊名称:JOURNAL OF GENERAL VIROLOGY ( 影响因子:3.891; 五年影响因子:3.719 )
ISSN: 0022-1317
年卷期: 2018 年 99 卷 11 期
页码:
收录情况: SCI
摘要: Garlic virus X (GarVX) encodes a 15 kDa cysteine-rich protein (CRP). To investigate the function(s) of p15, its subcellular localization, role as a symptom determinant and capacity to act as a viral suppressor of RNA silencing (VSR) were analysed. Results showed that GFP-tagged p15 was distributed in the cytoplasm, nucleus and nucleolus. Expression of p15 from PVX caused additional systemic foliar malformation and led to increased accumulation of PVX, showing that p15 is a virulence factor for reconstructed PVX-p15. Moreover, using a transient agro-infiltration patch assay and a Turnip crinkle virus (TCV) movement complementation assay, it was demonstrated that p15 possesses weak RNA silencing suppressor activity. Removal of an amino acid motif resembling a nuclear localization signal (NLS) prevented p15 from accumulating in the nucleus but did not abolish its silencing suppression activity. This study provides the first insights into the multiple functions of the GarVX p15 protein.
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