SLAF-Based Construction of a High-Density Genetic Map and Its Application in QTL Mapping of Carotenoids Content in Citrus Fruit
文献类型： 外文期刊
第一作者： Zheng, Xiongjie
作者： Zheng, Xiongjie;Tang, Yuqing;Ye, Junli;Pan, Zhiyong;Tan, Meilian;Xie, Zongzhou;Chai, Lijun;Xu, Qiang;Deng, Xiuxin;Fraser, Paul D.;Tan, Meilian
作者机构：
关键词： Citrus; SLAF; high-density genetic map; carotenoids; quantitative trait loci (QTL)
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 3 期
页码：
收录情况： SCI
摘要： Carotenoids are important antioxidant components in the human diet. To develop carotenoid-rich agricultural products by genetic intervention, understanding the genetic basis of carotenoids variation is essential. In this study, we constructed a high-density integrated genetic map with 3817 molecular markers using specific locus amplified fragment (SLAF) sequencing from a C. reticulata x P. trifoliata F-1 pseudotestcross population. A total of 17 significant quantitative trait loci (QTLs) distributed on Chromosomes (Chr) 2, 3, 5, 6, and 9 were detected to determine the carotenoid variation in the population. In particular, three QTL colocalizations for multiple carotenoid constituents were observed on Chr 2, 3, and 9, one of which was located on Chr2:34,654,608-35430715 accounted for 20.1-25.4% of the variation of luteoxanthin, auroxanthin, lutein, violaxanthin, and total carotenoid content. Overall, this study provides a genetic foundation for marker-assisted selection (MAS) breeding of nutritionally enhanced citrus fruit.
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