Goose MAVS functions in RIG-I-mediated IFN-beta signaling activation

文献类型： 外文期刊

第一作者： Sun, Yingjie

作者： Sun, Yingjie;Zheng, Hang;Wu, Wei;Rehman, Zaib Ur;Liao, Ying;Meng, Chunchun;Qiu, Xusheng;Tan, Lei;Song, Cuiping;Yu, Shengqing;Ding, Chan;Mao, Xuming;Xu, Lei

作者机构：

关键词： Goose; MAVS; Newcastle disease virus (NDV); Innate immunity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2019 年 93 卷

页码：

收录情况： SCI

摘要： Mitochondrial antiviral-signaling protein (MAVS) is an essential adaptor protein in retinoic acid-inducible gene I (RIG-I)-mediated antiviral innate immunity in mammals. In this study, the goose MAVS gene (goMAVS) was identified. The 2019 bp-long goMAVS exhibits 96.2% amino acid similarity compared to the predicted goMAVS. Quantitative real-time polymerase chain reactions showed that goMAVS mRNA was widely expressed in different tissues. The overexpression of goMAVS in goose embryo fibroblast cells up-regulated the mRNA levels of goose interferon-stimulated genes. We concluded that MAVS mediates the activation of type I interferon (IFN) pathway in a species-specific manner. We further demonstrated that a CARD-like domain, transmembrane domain and two previously unidentified domains of goMAVS were essential for the activation of type I IFN pathway. GoMAVS inhibited Newcastle disease virus replication by activating type I IFN pathways, especially at the early stages of infection. Finally, the interaction between goMAVS and goose RIG-I was confirmed. The CARD domain of goMAVS plays a vital role in the interaction. Together, we identified goMAVS as a goRIG-I interactive protein and concluded that goMAVS is involved in the activation of type I IFN pathways in goose cells.

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