A novel glutathione S-transferase gene from sweetpotato, IbGSTF4, is involved in anthocyanin sequestration

文献类型： 外文期刊

第一作者： Kou, Meng

作者： Kou, Meng;Liu, Ya-ju;Li, Zong-yun;Li, Qiang;Ma, Dai-fu;Kou, Meng;Liu, Ya-ju;Zhang, Yun-gang;Tang, Wei;Yan, Hui;Wang, Xin;Chen, Xiao-guang;Su, Zai-xing;Li, Qiang;Ma, Dai-fu;Arisha, Mohamed Hamed

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关键词： Sweetpotato; Glutathione S-transferase; Anthocyanin transportation

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2019 年 135 卷

页码：

收录情况： SCI

摘要： Anthocyanins are synthesized by multi-enzyme complexes localized at the cytoplasmic surface of the endoplasmic reticulum (synthesis site), and transported to the destination site, the vacuole. Three mechanisms for the vacuolar accumulation of anthocyanin in plant species have been proposed. Previous studies have indicated that glutathione S-transferase (GST) genes from model and ornamental plants are involved in anthocyanin transportation. In the present study, an anthocyanin-related GST, IbGSTF4, was identified and characterized based on transcriptome results. Phylogenetic analysis revealed that IbGSTF4 was most closely correlated to PhAN9 and CkmGST3, the anthocyanin-related GST of Petunia hybrida and Cyclamen. Furthermore, the expression analysis revealed that IbGSTF4 is strongly expressed in pigmented tissues, when compared to green organs, which is in agreement to the ability to correlate with anthocyanin accumulation. A GST activity assay uncovered that the IbGST4 protein owned similar activities with the GST family. Furthermore, the molecular functional complementation of Arabidopsis thaliana mutant tt19 demonstrated that IbGSTF4 might play a vital role in the vacuole sequestration of anthocyanin in sweetpotato. Moreover, the dual luciferase assay revealed that the LUC driven by the promoter of IbGSTF4 could not be directly activated by IbMYB1, suggesting that the regulatory mechanism of anthocyanin accumulation and sequestration in sweetpotato was intricate.

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