Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

文献类型： 外文期刊

第一作者： Xie, Li-hua

作者： Xie, Li-hua;Quan, Xin;Yang, Yan-yan;Sun, Run-hong;Xia, Ming-cong;Xue, Bao-guo;Wu, Chao;Han, Xiao-yun;Yang, Li-rong;Xue, Ya-nan

作者机构：

关键词： geNorm; housekeeping gene; Triticum aestivum; TUB beta

期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）

ISSN： 1598-2254

年卷期： 2019 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. TUB beta was identified as the most stable reference gene. Furthermore, the exopoly-galacturonase gene (ExoPG) was selected to verify the reliability of TUB beta expression. The expression profile of ExoPG assessed using TUB beta agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

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