Induced expression of small heat shock proteins is associated with thermotolerance in female Laodelphax striatellus planthoppers

文献类型： 外文期刊

第一作者： Wang, Lihua

作者： Wang, Lihua;Zhang, Yueliang;Pan, Lei;Han, Yangchun;Niu, Hongtao;Shan, Dan;Fang, Jichao;Wang, Qin;Hoffmann, Ary

作者机构：

关键词： Laodelphax striatellus; Thermotolerance; sHsps; Expression; Stress

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN： 1355-8145

年卷期： 2019 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Insects are often exposed to high temperature stress in natural environments, but the mechanisms involved in thermotolerance in many insect groups like Hemiptera are not well known. To explore possible mechanisms of thermotolerance in the hemipteran pest Laodelphax striatellus, which damages rice through direct feeding and viral transmission, small heat shock proteins (sHsps) implicated in thermotolerance in other insect groups were identified. The seven sHsps identified have a conserved alpha crystallin domain, a variable N-terminal region, and shared relative low identities to each other. Three of the sHsp genes (LsHsp20.5, LsHsp21.5, and LsHsp21.6) exhibited higher basal expression than the other four genes but showed weak or no heat-induced expression. The other four genes (LsHsp20.1, LsHsp21.2, LsHsp21.4, and LsHsp22.0) were induced up to 3306-fold by heat. Injection of dsRNA indicated that expression of these sHsps was associated with thermotolerance, and Escherichia coli transformed with LsHsp21.2 and LsHsp20.1 showed relatively higher thermotolerance. These results point to an important functional role of these sHsps for thermotolerance in L. striatellus.

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