Bioinformatic analysis of the complete genome sequence of Pectobacterium carotovorum subsp. brasiliense BZA12 and candidate effector screening

文献类型： 外文期刊

第一作者： Huang, Yufei

作者： Huang, Yufei;Liu, Changyuan;Wang, Hui;Guan, Tianshu;Liu, Li;Yu, Shuyi

作者机构：

关键词： P; carotovorum subsp; brasiliense; Complete genome sequence; Effector

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期： 2019 年 101 卷 1 期

页码：

收录情况： SCI

摘要： Pectobacterium carotovorum subsp. brasiliense (Pcb) is a gram-negative, plant pathogenic bacterium of the soft rot Enterobacteriaceae (SRE) family. We present the complete genome sequence of Pcb strain BZA12, which reveals that Pcb strain BZA12 carries a single 4,924,809bp chromosome with 51.97% GC content and comprises 4508 predicted protein-coding genes. Gene annotation of these genes utilized GO, KEGG, and COG databases. In comparison with three closely related soft-rot pathogens, strain BZA12 has 3797 gene families, among which 3107 gene families are identified as orthologous with those of both P. carotovorum subsp. carotovorum PCC21 and P. carotovorum subsp. odoriferum BCS7, as well as 36 putative Unique Gene Families. We selected five putative effectors from the BZA12 genome and transiently expressed them in Nicotiana benthamiana. Candidate effector A12GL002483 was localized in the cell nucleus and induced cell death. This study provides a foundation for a better understanding of the genomic structure and function of Pcb, particularly in the discovery of potential pathogenic factors and for the development of more effective strategies against this pathogen.

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