Development of a qPCR Protocol to Detect the Cotton Bacterial Blight Pathogen, Xanthomonas citri pv. malvacearum, from Cotton Leaves and Seeds

文献类型： 外文期刊

第一作者： Wang, Xiao-Qiang

作者： Wang, Xiao-Qiang;Deng, Peng;Jia, David;Lu, Shi-En;Wang, Xiao-Qiang;Allen, Tom W.;Wang, Hui;Peterson, Daniel G.;Nichols, Robert L.;Liu, Aixin;Li, Xiang-Dong

作者机构：

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2019 年 103 卷 3 期

页码：

收录情况： SCI

摘要： Bacterial blight, historically a seed-borne disease of cotton (Gossypium hirsutum) is caused by Xanthomonas cirri pv. malvacearum, resulted in significant economic losses prior to development of resistant varieties and implementation of acid-delinting of planting seed. Periodic outbreaks have been associated with seed since the early twentieth century; of note, the disease has experienced a resurgence since 2011. Effective management of bacterial blight is dependent on accurate diagnosis and detection of the pathogen. Currently, detection of X. cirri pv. malvacearum is performed by time-consuming microbiological methods. In this study, a novel and sensitive TaqMan-based qPCR protocol was developed to test for X. citri pv. malvacearum in cotton plant tissue. The primers developed are specific to five races of X. citri pv. malvacearum, but not to other Xanthomonas species or cotton-associated nonpathogenic bacteria. The efficiency of this assay was evaluated on artificially inoculated cotton leaves and seed, on naturally infected cotton leaves, and on bolls and seed originating from bacterial blight symptomatic bolls. The protocol's efficiency from artificially inoculated plant tissue was 10(2) copies g(-1) and 37 copies from 1 g seed for leaves and seed, respectively. In addition, X. citri pv. malvacearum was detected from 94% of the seed samples originating from blight symptomatic bolls. The qPCR protocol provides a rapid and accurate method for diagnosis and detection of bacterial blight and offers a tool for monitoring X. citri pv. malvacearum and potentially reducing its spread in seed.

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