Virus-induced Phytoene Desaturase (PDS) Gene Silencing Using Tobacco Rattle Virus in Lilium x formolongi

文献类型： 外文期刊

第一作者： Xu, Hua

作者： Xu, Hua;Xu, Leifeng;Yang, Panpan;Cao, Yuwei;Tang, Yuchao;He, Guoren;Yuan, Suxia;Lei, Jingyi;Ming, Jun;Xu, Hua;Yang, Panpan

作者机构：

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）

ISSN： 2095-9885

年卷期： 2019 年 5 卷 1 期

页码：

收录情况： SCI

摘要： Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco rattle virus (TRV)-based VIGS of a PHYTOENE DESATURASE (PDS) ortholog (LhPDS) can be achieved in Lilium x formolongi seedlings, with a survival rate of 92%, using the inoculation method of rubbing plus injection. Compared with untreated and mock-treated seedlings, the photobleached leaf phenotype of silenced plants significantly correlates with down-regulation of endogenous LhPDS (P <= 0.05). In addition, the silencing phenomenon can be observed in the growing points of plants, indicating that systemic viral infection was achieved using the protocol. The results indicate that TRV-based VIGS can be used to characterize gene function in Lilium x formolongi. This work lays the foundation for gene function analysis and molecular breeding in Lilium spp.

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