PacBio full-length transcriptome analysis reveals the role of tRNA-like structures in RNA processing

文献类型： 外文期刊

第一作者： Hu, Yanping

作者： Hu, Yanping;Yan, Haohao;Gao, Shan;Hu, Yanping;Li, Wenjing;Yan, Shuangyong;Su, Jingping;Cui, Zhongqiu;Li, Junling;Wang, Shengjun;Sun, Yue

作者机构：

关键词： TIS; TTS; polyA-like site; tRNA-like site; Inverted repeat

期刊名称：CELLULAR SIGNALLING （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 0898-6568

年卷期： 2025 年 125 卷

页码：

收录情况： SCI

摘要： Background: Mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA) are distinct from nuclear DNA (nuDNA) in a eukaryotic cell. Animal mitochondria transcribe a single primary transcript that carries all genes from a DNA strand; In contrast, plant mitochondria and chloroplasts produce multiple primary transcripts, with each transcript carrying several genes. How primary transcripts of plant mtDNA and cpDNA are processed into mature RNAs is still unknown. Results: In the present study, we employed PacBio's full-length transcriptome data to characterize the transcription of Arabidopsis thaliana mtDNA, providing a more comprehensive and precise understanding. The primary findings included 20 novel mitochondrial (mt) RNAs of A. thaliana, transcripts carrying single introns or exons, long mt and chloroplast (cp) tRNAs with intricate secondary structures, and the role of tRNA-like structures in RNA processing. The gene of No. 20 novel mt RNA and its paralog on chromosome 2 of A. thaliana were assigned locus IDs ATMG01335 and AT2G07811. Conclusions: According to our upgraded "mitochondrial cleavage" model, tRNA-like structures serve as "punctuation" marks for RNA processing, akin to the role of tRNAs. Both tRNA-like structures and tRNAs collaborate for RNA processing in plant mitochondria and chloroplasts.

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