Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli

文献类型： 外文期刊

第一作者： Liu, Changqing

作者： Liu, Changqing;Zhang, Kai;Cao, Wenyan;Zhang, Ge;Chen, Guoqiang;Yang, Haiyan;Xian, Mo;Zhang, Haibo;Liu, Changqing;Zhang, Kai;Cao, Wenyan;Zhang, Ge;Chen, Guoqiang;Yang, Haiyan;Xian, Mo;Zhang, Haibo;Zhang, Ge;Chen, Guoqiang;Wang, Qian;Liu, Haobao

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关键词： 2-Phenylethanol; Enterobacter sp; CGMCC 5087; 2-Keto acid decarboxylase; Alcohol dehydrogenase; Phenylpyruvate pathway; Metabolic engineering

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN： 1754-6834

年卷期： 2018 年 11 卷

页码：

收录情况： SCI

摘要： Background2-Phenylethanol (2-PE) is a higher aromatic alcohol that is widely used in the perfumery, cosmetics, and food industries and is also a potentially valuable next-generation biofuel. In our previous study, a new strain Enterobacter sp. CGMCC 5087 was isolated to produce 2-PE from glucose through the phenylpyruvate pathway.ResultsIn this study, candidate genes for 2-PE biosynthesis were identified from Enterobacter sp. CGMCC 5087 by draft whole-genome sequence, metabolic engineering, and shake flask fermentation. Subsequently, the identified genes encoding the 2-keto acid decarboxylase (Kdc) and alcohol dehydrogenase (Adh) enzymes from Enterobacter sp. CGMCC 5087 were introduced into E. coli BL21(DE3) to construct a high-efficiency microbial cell factory for 2-PE production using the prokaryotic phenylpyruvate pathway. The enzymes Kdc4427 and Adh4428 from Enterobacter sp. CGMCC 5087 showed higher performances than did the corresponding enzymes ARO10 and ADH2 from Saccharomyces cerevisiae, respectively. The E. coli cell factory was further improved by overexpressing two upstream shikimate pathway genes, aroF/aroG/aroH and pheA, to enhance the metabolic flux of the phenylpyruvate pathway, which resulted in 2-PE production of 260mg/L. The combined overexpression of tktA and ppsA increased the precursor supply of erythrose-4-phosphate and phosphoenolpyruvate, which resulted in 2-PE production of 320mg/L, with a productivity of 13.3mg/L/h.ConclusionsThe present study achieved the highest titer of de novo 2-PE production of in a recombinant E. coli system. This study describes a new, efficient 2-PE producer that lays foundation for the industrial-scale production of 2-PE and its derivatives in the future.

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