Covalent modification of beta-lactoglobulin by (-)-epigallocatechin-3-gallate results in a novel antioxidant molecule

文献类型： 外文期刊

第一作者： Tao, Fei

作者： Tao, Fei;Xiao, Chaogeng;Chen, Wenwei;Zhang, Yongyong;Pan, Jiarong;Jia, Zhenbao

作者机构：

关键词： beta-Lactoglobulin; (-)-Epigallocatechin-3-gallate; Modification; Antioxidant

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2019 年 126 卷

页码：

收录情况： SCI

摘要： beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. The EGCG-modified beta-lg was characterized by SDS-PAGE, MALDI-TOF MS and intrinsic fluorescence spectroscopy. The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. SDS-PAGE and MALDI-TOF MS results indicated the dimerization of beta-lg after EGCG modification. Intrinsic fluorescence spectra suggested that EGCG modification caused an alteration in the conformational structure of beta-lg. The results demonstrated that EGCG-modified beta-lg possessed great antioxidant potential in terms of scavenging DPPH radical and chelating ferrous ion. Furthermore, the EGCG-modified beta-lg showed a protective effect against LDL peroxidation. The results indicate that EGCG-modified beta-lg could provide significant health benefits as an antioxidant. (C) 2019 Published by Elsevier B.V.

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