Methamidophos Influences Midgut Proteinase Activity and Subcellular Structures in the Wolf Spider Pardosa pseudoamulata (Araneae: Lycosidae)

文献类型： 外文期刊

第一作者： Wang, Juan

作者： Wang, Juan;Peng, Yuande;Wang, Zhi;Wang, Juan;Yang, Huilin;Li, Qinjin;Luo, Lin;Li, Qinjin;Wang, Zhi;Song, Qisheng;Stanely, David

作者机构：

关键词： PQCI sensor; Pardosa pseudoamulata; methamidophos; proteinase

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN： 0022-0493

年卷期： 2019 年 112 卷 1 期

页码：

收录情况： SCI

摘要： A piezoelectric quartz crystal impedance (PQCI) sensor was used to investigate influences of the insecticide methamidophos on proteinase activity in midguts of the wolf spider, Pardosa pseudoamulata (Araneae: Lycosidae). Results from PQCI indicated that low-concentration dose methamidophos (0.008%) can activate the proteinase but high-concentration dose methamidophos (0.016-0.032%) can inhibit the enzyme activity. The changes in subcellular structure of spider midgut cells were also observed. Electron micrographs of spider midgut epithelial cells showed that the low-dose methamidophos did not visibly impact the structure of these cells. Conversely, high-concentration dose methamidophos led to severe changes in the cell structure, including the karyotheca dissolved, the nucleolus, and the endoplasmic reticulum disappeared. These may contribute to changes in proteinase activity of spider. This work documents a feasible method for rapid and reliable detection of proteinase activity.

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