An R3-MYB gene of Phalaenopsis, MYBx1, represses anthocyanin accumulation

文献类型： 外文期刊

第一作者： Fu, Zhenzhu

作者： Fu, Zhenzhu;Wang, Limin;Shang, Hongquan;Dong, Xiaoyu;Jiang, Hui;Zhang, Jing;Wang, Huijuan;Li, Yanmin;Yuan, Xin;Meng, Siyu;Gao, Jie;Feng, Naixi;Zhang, Hechen

作者机构：

关键词： Anthocyanin; Gene function; Phalaenopsis; Transcription factor

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

ISSN： 0167-6903

年卷期： 2019 年 88 卷 2 期

页码：

收录情况： SCI

摘要： Anthocyanins are secondary metabolites that are involved in providing flower petal pigmentation. In plants, anthocyanin synthesis is regulated positively by a conserved MYB-bHLH-WD40 (MBW) complex and negatively by repressors. We isolated a repressor of anthocyanin synthesis, MYBx1, from a moth orchid Phalaenopsis cv. Big Chili. MYBx1 encodes a typical R3-MYB protein and exhibited diverse expression patterns in four Phalaenopsis cultivars, Big Chili, Fuller's Sunset, Sogo Yukidian V3, and Sogo Lit-Sunny. A yeast two-hybrid assay analysis indicated that MYBx1 can also participate in the MBW complex by interacting with the transcription factors ANTHOCYANIN (AN) 1 and AN11. Over-expressing MYBx1 in the petunia hybrid M1xR27 resulted in reduced accumulations of flower petal pigments through the repression of anthocyanin activities. A quantitative real-time PCR analysis of the expression patterns of anthocyanin synthesis-related structural genes in the transgenic petunia flower petals showed that the expression levels of chalcone synthase, flavanone 3-hydroxylase, and dihydroflavonol reductase genes were considerably down-regulated. However, no significant changes in the expression levels of the MBW complex members, AN1, AN2, and AN11, were observed. This study improves our understanding of anthocyanin synthesis-related regulatory networks in plants and also provides an important foundation for the further genetic engineering of ornamental plant flower colors.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序