Salicylic acid involved in chilling-induced accumulation of calycosin-7-O--d-glucoside in Astragalus membranaceus adventitious roots

文献类型： 外文期刊

第一作者： Liu, Junpin

作者： Liu, Junpin;Lv, Shuang;Bao, Rui;Yuan, Yuan;Wu, Songquan;Quan, Xueli;Lan, Xi

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关键词： Astragalus membranaceus; Adventitious roots; Calycosin-7-O--d-glucoside; Gene expression; Salicylic acid; Chilling

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN： 0137-5881

年卷期： 2019 年 41 卷 7 期

页码：

收录情况： SCI

摘要： Calycosin and calycosin-7-O--d-glucoside (CG) are major isoflavonoids in Astragalus membranaceus and have multiple beneficial activities. Adventitious roots (ARs) are becoming attractive resources to obtain biologically active compounds. Salicylic acid (SA) is an important endogenous phytohormone, which is involved in the regulation of biotic and abiotic stresses. However, little is known about the potential role of SA on isoflavonoid accumulations under chilling stress. In the present study, calycosin was found to accumulate mostly in its glucosyl conjugate (CG) form in A. membranaceus ARs (AMARs). Compared to control conditions (25 degrees C), chilling (5 degrees C) induced the accumulation of CG, which was confirmed by increased expression levels of gene-encoding enzymes in the CG biosynthetic pathway. Furthermore, chilling triggered the accumulation of SA prior to CG accumulation. In addition, the inhibition of SA biosynthesis with paclobutrazol (PAC) in chilling-exposed AMARs suppressed the accumulation of CG and gene expressions, while exogenous addition of SA to PAC-treated AMARs restored CG content and gene expressions. These results indicated that in AMARs, SA involved in chilling-induced CG accumulation by regulating the expression levels of genes in the CG biosynthetic pathway.

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