Zero-valent Fe and MnSO4 collectively stimulate the degradation-related functionalities in the bacterial secretome affecting the efficient degradation of lignocellulose during composting

文献类型： 外文期刊

第一作者： Lu, Xiaolin

作者： Lu, Xiaolin;Lin, Shangyu;Yang, Yinfeng;Li, Qunliang;Zhang, Qiqiang;Huang, Rongchun;Mo, Naiguo;Liang, Shasha;Qin, Guangsheng

作者机构：

关键词： Lignocellulose; Composting; Metagenomics; Metaproteomics; Bacterial secretome

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）

ISSN： 1385-8947

年卷期： 2025 年 518 卷

页码：

收录情况： SCI

摘要： The degradation of lignocellulose in composting is driven by multiple factors. The auxiliary activity (AA) and glycosyl hydrolase (GH) families are key contributors to lignocellulose degradation, though how the functional materials regulate their functions in lignocellulose degradation during composting remains unclear. This study used metagenomics and metaproteomics to determine how zero-valent Fe and MnSO4 molecularly influence lignocellulose degradation during composting. The combined addition of zero-valent Fe and MnSO4 (CMF treatment) had the highest temperature (76.60 degrees C) and remained over 70 degrees C for 8 days. The CMF treatment stimulated extracellular enzymatic activities the most and promoted more reactive oxygen species generation during the thermophilic period. Compared to the initial period of composting, CMF ultimately resulted in the highest degradation rates for total organic carbon (19.94 %), cellulose (61.48 %), hemicellulose (25.80 %), and lignin (29.60 %). Metagenomic analysis indicated that the relative abundances of the major lignocellulolytic bacteria and functional genes were effectively increased in the thermophilic period of CMF treatment compared to other treatments. Random forest and variation partitioning analysis revealed that Actinomycetota and extracellular enzymatic activities had the strongest contributions to lignocellulose biodegradation. Metaproteomics and model analysis verified that high temperature significantly stimulated the functional expression of AA10, GH4, and GH6 families within the bacterial secretome and built the most active network for synergistically catalyzing lignocellulose degradation. Our findings provide new insights for efficient degradation of lignocellulose mediated by selectively regulating bacterial functional expression during composting.

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