Immune Enhancement of Nanoparticle-Encapsulated Ginseng Stem-Leaf Saponins on Porcine Epidemic Diarrhea Virus Vaccine in Mice
文献类型： 外文期刊
第一作者： Su, Fei
作者： Su, Fei;Xu, Lihua;Li, Junxing;Yu, Bin;Ye, Shiyi;Yuan, Xiufang;Xue, Yin;Xu, Wei
作者机构：
关键词： ginseng stem-leaf saponins; nanoparticles; mucosal adjuvant; porcine epidemic diarrhea virus
期刊名称：VACCINES （ 影响因子：7.8； 五年影响因子：7.4 ）
ISSN：
年卷期： 2022 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea virus (PEDV) causes severe enteric disease in pigs, particularly neonatal piglets. Current vaccines do not provide complete protection against PEDV. Ginseng stem-leaf saponins (GSLS), a promising oral adjuvant candidate, can improve intestinal immune responses in poultry and mice. However, its low stability limits further use. Poly lactic-co-glycolic acid (PLGA), a biocompatible and biodegradable nanoparticle, has been widely used in biomedicine for stable and targeted drug delivery. In this study, we developed GSLS-PLGA nanoparticles (GSLS-NPs) and evaluated the mucosal adjuvant efficacy in vitro and in vivo. GSLS-NPs significantly enhanced antigen internalization and pro-inflammatory cytokine secretion by DC2.4 cells. Mice orally administered GSLS-NPs before intramuscular inoculation generated CD11b(+)CD8 alpha(-) and CD11b(-)CD103(+) dendritic cells in the spleen and draining mesenteric lymph nodes, respectively, which are the types mainly responsible for antigen presentation. Additionally, enhanced neutralizing and non-neutralizing antibody responses and expanded activities of specific effector and memory CD4(+) and CD8(+) T cells were also observed in mice immunized with PEDV vaccines plus GSLS-NPs compared to mice receiving the vaccines alone. Furthermore, GSLS-NPs showed a good safety profile and presented great advantages over GSLS aqueous solution. Collectively, our results highlight the potential of GSLS-NPs as a mucosal adjuvant and provide an attractive vaccination strategy for combatting PEDV. Further study is required to evaluate the efficacy of this mucosal adjuvant in swine.
分类号：
- 相关文献
作者其他论文 更多>>
-
Selenium-rich Lactobacillus plantarum alleviates salinity stress in Cyprinus carpio: Growth performance, oxidative stress, and immune and inflammatory responses
作者：Che, Xinghua;Geng, Longwu;Zhang, Qing;Wei, Haijun;Xu, Wei;Shang, Xinchi;Che, Xinghua;Geng, Longwu;Zhang, Qing;Wei, Haijun;Xu, Wei;Shang, Xinchi;He, Hailong;Shang, Xinchi
关键词：Selenium -rich Lactobacillus plantarum; Salinity stress; Antioxidants; Inflammatory responses; Cyprinus carpio
-
Proteomics and metabolomics analysis of American shad (Alosa sapidissima) liver responses to heat stress
作者：Luo, Mingkun;Zhu, Wenbin;Fu, Jianjun;Miao, Linghong;Dong, Zaijie;Feng, Bingbing;Xu, Wei;Liang, Zhengyuan;Dong, Zaijie
关键词：Alosa sapidissima; Liver; Heat stress; Proteome; Metabolome
-
Analysis revealed the molecular mechanism of oxidative stress-autophagy-induced liver injury caused by high alkalinity: integrated whole hepatic transcriptome and metabolome
作者：Shang, Xinchi;Geng, Longwu;Wei, Hai jun;Liu, Tianqi;Che, Xinghua;Li, Wang;Shi, Xiao dan;Xu, Wei;Shang, Xinchi;Geng, Longwu;Wei, Hai jun;Liu, Tianqi;Che, Xinghua;Li, Wang;Xu, Wei;Shang, Xinchi;Li, Jianhong;Liu, Yuhao;Teng, Xiaohua
关键词：high alkalinity stress; MiRNA-mRNA network; metabolomics; autophagy; miR-140-5p-ULK2 axis
-
Selenium-enriched Lactobacillus plantarum alleviate of high alkalinity-induced microbiota-gut-blood systems affect by improving the gut microbiota
作者：Shang, Xinchi;Xing, Lu;Li, Jian Hong;Shang, Xinchi;Geng, Longwu;Wei, Haijun;Che, Xinghua;Xing, Meiqi;Xu, Wei;Shang, Xinchi;Geng, Longwu;Wei, Haijun;Che, Xinghua;Xing, Meiqi;Xu, Wei
关键词：Se-enriched L. plantarum; High alkalinity; Gut microbial; Tight junction protein; LPS
-
Multifunctional Dual Network Hydrogel Loaded with Novel Tea Polyphenol Magnesium Nanoparticles Accelerates Wound Repair of MRSA Infected Diabetes
作者：Hu, Xulin;Qian, Zhiyong;Hu, Xulin;Qian, Zhiyong;Hu, Xulin;Wang, Chun;Wang, Yao;Hu, Xulin;Wang, Chun;Wang, Yao;He, Jian;Qiao, Liang;Wang, Chun;Wang, Yao;Yu, Rongxin;Xu, Wei;Zhang, Xiangchun;Wang, Fan;Yang, Shuhao
关键词：diabetes wound healing; double network hydrogel; magnesium nanoparticles; MRSA biofilm; tea polyphenol
-
The Prediction of Clinical Mastitis in Dairy Cows Based on Milk Yield, Rumination Time, and Milk Electrical Conductivity Using Machine Learning Algorithms
作者：Tian, Hong;Zhou, Xiaojing;Zhou, Xiaojing;Zhao, Zixuan;Wang, Hao;Xu, Chuang;Deng, Zhaoju;Xu, Wei
关键词：cow; mastitis; prediction; rumination; electrical conductivity of milk; machine learning
-
Ascorbic Acid Improves Tomato Salt Tolerance by Regulating Ion Homeostasis and Proline Synthesis
作者：Chen, Xianjun;Han, Hongwei;Cong, Yundan;Li, Xuezhen;Zhang, Wenbo;Cui, Jinxia;Xu, Wei;Pang, Shengqun;Liu, Huiying;Chen, Xianjun;Han, Hongwei
关键词：ascorbic acid; ion homeostasis; osmotic balance; salt stress; tomato
