Isolation and characterization of a rice mutant with narrow and rolled leaves

文献类型： 外文期刊

第一作者： Wu, Chao

作者： Wu, Chao;Fu, Yaping;Hu, Guocheng;Si, Huamin;Cheng, Shihua;Liu, Wenzhen

作者机构：

关键词： Cellulose synthase-like gene;Map-based cloning;Narrow and rolled leaves;NRL1;Rice (Oryza sativa L.)

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Appropriate leaf shape has proved to be useful in improving photosynthesis and increasing grain yield. To understand the molecular mechanism of leaf morphogenesis, we identified a rice mutant nrl1, which was characterized by a phenotype of narrow and rolled leaves. Microscopic observation showed that the mutation significantly decreased the number of vascular bundles of leaf and stem. Genetic analysis revealed that the mutation was controlled by a single nuclear-encoded recessive gene. To isolate the nrl1 gene, 756 F_2 and F_3 mutant individuals from a cross of the nrl1 mutant with Longtepu were used and a high-resolution physical map of the chromosomal region around the nrl1 gene was made. Finally, the gene was mapped in 16.5 kb region between marker RL21 and marker RL36 within the BAC clone OSJNBa0027H05. Cloning and sequencing of the target region from the mutant showed that there was a 58 bp deletion within the second exon of the cellulose synthase-like D4 gene (TIGR locus Os12g36890). The nrl1 mutation was rescued by transformation with the wild-type cellulose synthase-like D4 gene. Accordingly, the cellulose synthase-like D4 gene was identified as the NRL1 gene. NRL1 was transcribed in various tissues and was mainly expressed in panicles and internodes. NAL7 and SLL1 were found to be upregulated, whereas OsAGO7 were downregulated in the nrl1 mutant. These findings suggested that there might be a functional association between these genes in regulating leaf development.

分类号： Q94

  • 相关文献

[1]A B-lectin receptor kinase gene conferring rice blast resistance. Chen, Xuewei,Shang, Junjun,Chen, Dexi,Lei, Cailin,Zou, Yan,Zhai, Wenxue,Liu, Guozhen,Xu, Jichen,Ling, Zhongzhuan,Cao, Gang,Ma, Bingtian,Wang, Yuping,Zhao, Xianfeng,Li, Shigui,Zhu, Lihuang. 2006

[2]Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Hu, Jiang,Zhu, Li,Zeng, Dali,Gao, Zhenyu,Guo, Longbiao,Fang, Yunxia,Zhang, Guangheng,Dong, Guojun,Yan, Meixian,Liu, Jian,Qian, Qian.

[3]Importance of the rice TCD9 encoding alpha subunit of chaperonin protein 60 (Cpn60 alpha) for the chloroplast development during the early leaf stage. Jiang, Quan,Mei, Jie,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Xu, Jian-Long,Zhang, Jian-Hui,Teng, Sheng. 2014

[4]The rice TCD11 encoding plastid ribosomal protein S6 is essential for chloroplast development at low temperature. Wang, Wen-Juan,Zheng, Kai-Lun,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Gong, Xiao-Di,Xu, Jian-Long,Xu, Jian-Long,Huang, Ji-Rong.

[5]Cloning and functional analysis of pale-green leaf (PGL10) in rice (Oryza sativa L.). Yang, Yao-Long,Xu, Jie,Zeng, Yong-Jun,Shi, Qing-Hua,Yang, Yao-Long,Xu, Jie,Liu, Hui-Juan,Zheng, Ting-Ting,Zhang, Guang-Heng,Hu, Jiang,Guo, Long-Biao,Qian, Qian,Zeng, Da-Li,Rao, Yu-Chun.

[6]Characterization and gene cloning of the rice (Oryza sativa L.) dwarf and narrow-leaf mutant dnl3. Shi, L.,Tang, S. Q.,Hu, P. S.,Wang, J. L.,Shi, L.,Wei, X. J.,Adedze, Y. M. N.,Sheng, Z. H.,Tang, S. Q.,Hu, P. S.. 2016

[7]The newly identified heat-stress sensitive albino 1 gene affects chloroplast development in rice. Qiu, Zhennan,Kang, Shujing,He, Lei,Zhao, Juan,Zhang, Sen,Hu, Jiang,Zeng, Dali,Zhang, Guangheng,Dong, Guojun,Gao, Zhenyu,Ren, Deyong,Chen, Guang,Guo, Longbiao,Qian, Qian,Zhu, Li. 2018

[8]Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.). Zhao, Shasha,Wang, Cuihong,Ma, Jian,Wang, Shuai,Tian, Peng,Wang, Jiulin,Cheng, Zhijun,Zhang, Xin,Guo, Xiuping,Lei, Cailin. 2016

[9]A point mutation in the zinc finger motif of RID1/EHD2/OsID1 protein leads to outstanding yield-related traits in japonica rice variety Wuyunjing 7. Hu, Shikai,Dong, Guojun,Xu, Jie,Su, Yan,Shi, Zhenyuan,Ye, Weijun,Li, Yuanyuan,Li, Gengmi,Zhang, Bin,Hu, Jiang,Qian, Qian,Zeng, Dali,Guo, Longbiao. 2013

[10]Map-Based Cloning of zb7 Encoding an IPP and DMAPP Synthase in the MEP Pathway of Maize. Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Wang, Zhen-Hua,Zhao, Yuan-Zeng. 2012

[11]A WUSCHEL-like homeobox gene, OsWOX3B responses to NUDA/GL-1 locus in rice. Wu, Kun,Qian, Qian,Zhang, Honglei,Wang, Yufeng,Peng, Yu,Han, Bin,Teng, Sheng,Hu, Fengyi,Wen, Lu.

[12]Dwarf 88, a novel putative esterase gene affecting architecture of rice plant. Gao, Zhenyu,Liu, Xiaohui,Feng, Qi,Han, Bin,Gao, Zhenyu,Qian, Qian,Yan, Meixian,Dong, Guojun,Liu, Jian.

[13]Map-based cloning of the ALK gene, which controls the gelatinization temperature of rice. Gao, ZY,Zeng, DL,Cui, X,Zhou, YH,Yan, M,Huang, D,Li, JY,Qian, Q.

[14]Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice. Liu, Wenzhen,Wu, Chao,Fu, Yaping,Hu, Guocheng,Si, Huamin,Zhu, Li,Luan, Weijiang,He, Zhengquan,Sun, Zongxiu,He, Zhengquan.

[15]SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice. Chen, Xifeng,Hao, Liang,Pan, Jianwei,Zheng, Xixi,Jin, Yang,Gu, Zhimin,Ma, Bojun,Chen, Xifeng,Jiang, Guanghuai,Zhai, Wenxue,Qian, Qian.

[16]Identification and characterization of SHORTENED UPPERMOST INTERNODE 1, a gene negatively regulating uppermost internode elongation in rice. Zhu, Li,Hu, Jiang,Zhu, Keming,Fang, Yunxia,Gao, Zhenyu,He, Yinghong,Zhang, Guangheng,Guo, Longbiao,Zeng, Dali,Dong, Guojun,Yan, Meixian,Liu, Jian,Qian, Qian.

[17]Young Seedling Stripe1 encodes a chloroplast nucleoid-associated protein required for chloroplast development in rice seedlings. Zhou, Kunneng,Ren, Yulong,Zhou, Feng,Wang, Ying,Ma, Weiwei,Wu, Fuqing,Zhang, Xin,Guo, Xiupin,Cheng, Zhijun,Wang, Jiulin,Lei, Cailin,Wan, Jianmin,Zhang, Long,Lyu, Jia,Wang, Yihua,Zhao, Shaolu,Zhang, Huan,Wang, Liwei,Wang, Chunming,Jiang, Ling,Wan, Jianmin,Li, Zefu.

[18]Map-based cloning of a spotted-leaf mutant gene OsSL5 in Japonica rice. Ge, Chang-wei,Pan, Jiang-jie,Zhang, Xiao-qin,Xue, Da-wei,Ge, Chang-wei,E, Zhi-guo,Pan, Jiang-jie,Zeng, Da-li,Dong, Guo-jun,Hu, Jiang,Xue, Da-wei,Jiang, Hua.

[19]Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death. Lin, D.,Jiang, Q.,Zheng, K.,Chen, S.,Zhou, H.,Gong, X.,Dong, Y.,Chen, S.,Zhou, H.,Teng, S.,Xu, J..

[20]Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.). Lee, Yunjoo,Lee, Gileung,Jang, Su,Kim, Backki,Woo, Mi-Ok,Koh, Hee-Jong,Lee, Yunjoo,Lee, Gileung,Jang, Su,Kim, Backki,Woo, Mi-Ok,Koh, Hee-Jong,Lee, Yunjoo,Lee, Gileung,Jang, Su,Kim, Backki,Woo, Mi-Ok,Koh, Hee-Jong,Choi, Min-Seon,Yoon, Mi-Ra,Piao, Rihua,Chin, Joong Hyoun. 2017

作者其他论文 更多>>

微信小程序