Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

文献类型： 外文期刊

第一作者： Chai, JF

作者： Chai, JF;Wu, ZM;Zhao, H;Laroche, A;Wang, HB

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关键词： subtracted AFLP;subtractive hybridization;AFLP;alien chromosome fragment;wheat

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

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收录情况： SCI

摘要： Amplified fragment length polymorphism (AFLP) is a DNA fingerprinting technique (Vos et al, 1995). This technique is based on the detection of genomic restriction fragments by PCR amplification. Fingerprints are produced without prior sequence knowledge using a limited set of generic primers. By combining the reliability of the RFLP technique and the power of the PCR technique, the AFLP technique is robust and reliable, allowing the researcher to simultaneously evaluate more than 50 potential polymorphisms on a single denaturing polyacrylamide gel. This technique has been used successfully for identification of markers linked to many genes of interest (Parker et al, 1998; Bai and Kolb, 1999; Haiti et al, 1999; Huang et al, 2000). However this technique is not problem-free. When using AFLP to search for molecular markers with near-isogenic lines or bulked segregant analysis, it is easy to observe that the majority of amplified fragments is ubiquitous. In order to get a closely linked marker, usually a large number of primer pairs are needed to be screened (Huang et al, 2000). If a specific fragment is the same length as a ubiquitous fragment or just near to a strong common band, it will be more difficult for the specific band to be identified. Up to now, these problems have been paid no attention to.

分类号： Q94

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