Integration of transcriptomics and non-targeted metabolomics reveals the underlying mechanism of follicular atresia in Chinese buffalo

文献类型： 外文期刊

第一作者： Cheng, Juanru

作者： Cheng, Juanru;Pan, Yu;Yang, Sufang;Wei, Yaochang;Lv, Qiao;Xing, Qinghua;Zhang, Ruimen;Sun, Le;Shi, Deshun;Deng, Yanfei;Qin, Guangsheng

作者机构：

关键词： Follicular atresia; Buffalo; Non-targeted metabolomics; LC-MS; Transcriptomics; Lipid metabolism

期刊名称：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.294； 五年影响因子：4.217 ）

ISSN： 0960-0760

年卷期： 2021 年 212 卷

页码：

收录情况： SCI

摘要： Follicular atresia is a complex physiological process, which results in the waste of follicles and oocytes from the ovary. Elucidating the physiological mechanism of follicular atresia will hopefully reverse the fate of follicles, thereby improve the reproductive efficiency of female animals. However, there are still many gaps to be filled during the follicular atresia process. In this study, we first comprehensively summarized and compared a variety of methods to classify Chinese buffalo follicles with different extent of atresia. Then follicular fluid and granulosa cells from the corresponding follicles with different extent of atresia were collected for non-targeted metabolomics and transcriptomics analysis, respectively. After the detection and analysis of 129 follicles, a reasonable classification standard was formed: on the basis of morphological classification, the relative concentrations of estradiol (E2) and progesterone (PROG) in the follicular fluid were determined, follicles with an estradiol-toprogesterone (E2/PROG) ratio >5 were classified as healthy follicles (HF), 1 <= E2/PROG <= 5 as early atretic follicles (EF) and E2/PROG <1 as late atretic follicles (LF). Correspondingly, follicles with granulosa cells apoptosis rate less than 15 % were divided into HF, 15%-25% were classified as EF and more than 25 % were classified as LF. The integration analysis of non-targeted metabolomics and transcriptomics highlights the following three aspects: (1) Atresia seriously damaged the lipid metabolism homeostasis of follicle, in which PPAR gamma play important roles. (2) Energy metabolism and nucleotide metabolism of atretic follicles were inhibited. (3) Bilirubin is involved in follicular atresia, and it may be the main force to prevent lipid peroxidation in follicular cells. In summary, results of this study provide new understanding of the molecular mechanisms of Chinese buffalo follicular atresia.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序